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Am. J. Trop. Med. Hyg., 81(2), 2009, pp. 223-227
Copyright © 2009 by The American Society of Tropical Medicine and Hygiene

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Very Low Levels of Genetic Variation in Natural Peridomestic Populations of the Chagas Disease Vector Triatoma sordida (Hemiptera: Reduviidae) in Southeastern Brazil

Fernando A. Monteiro*, José Jurberg, AND Cristiano Lazoski
Laboratório de Doenças Parasitárias, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil; Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil; Laboratório de Biodiversidade Molecular, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

Levels of genetic variation and population structure were determined for 181 Triatoma sordida insects from four populations of southeastern Brazil, through the analysis of 28 allozyme loci. None of these loci presented fixed differences between any pair of populations, and only two revealed polymorphism, accounting for low levels of heterozygosity (He = 0.027), and low genetic distances (D < 0.03) among populations. FST and Contingency Table results indicated the existence of genetic structure among populations (FST = 0.214), which were incompatible with the isolation by distance model (Mantel test: r = 0.774; P = 0.249).

Received December 30, 2008. Accepted for publication March 12, 2009.

Acknowledgments: We thank João F. Martins for technical assistance, and the personnel of the National Health Foundation in Brazil (FUNASA), for help during the field collections. Haydée Cunha and an anonymous referee made insightful comments that improved the manuscript. Agarose allozyme electrophoresis was carried out in the laboratory of Ricardo Lourenço-de-Oliveira.

Financial support: This work was supported by the Brazilian National Research Council, CNPq.

* Address correspondence to Fernando A. Monteiro, Laboratório de Doenças Parasitárias, sala 21A, Instituto Oswaldo Cruz, Fiocruz, Av. Brasil 4365, Rio de Janeiro, RJ, 21045-900, Brazil. E-mail: fam{at}ioc.fiocruz.br

Authors’ addresses: Fernando A. Monteiro, Laboratório de Doenças Parasitárias, sala 21A, Instituto Oswaldo Cruz, Fiocruz, Av. Brasil 4365, Rio de Janeiro, RJ, 21045-900, Brazil, Tel: +55 21 2562-1255, Fax: +55 21 2280-3470, E-mail: fam{at}ioc.fiocruz.br. José Jurberg, Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil, Tel: +55 21 2598-4518, Fax: +55 21 2560-7317, E-mail: jjurberg{at}ioc.fiocruz.br. Cristiano Lazoski, Laboratório de Biodiversidade Molecular, Departamento de Genética, Universidade Federal do Rio de Janeiro, CCS, Bloco A, sala A2-98, Ilha do Fundão, Rio de Janeiro, RJ, 21941-590, Brazil, Tel: +55 21 2562-6389, Fax: +55 21 2562-6333, E-mail: lazoski{at}acd.ufrj.br.






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Copyright © 2009 by the American Society of Tropical Medicine and Hygiene.