AJTMH ASTMH Job Mart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 81(1), 2009, pp. 23-26
Copyright © 2009 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Loaiza, J.
Right arrow Articles by Conn, J. E.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Loaiza, J.
Right arrow Articles by Conn, J. E.
Right arrowPubmed/NCBI databases
*Protein
*Substance via MeSH

SHORT REPORT


Anopheles darlingi (Diptera: Culicidae) in Panama

Jose Loaiza, Marilyn Scott, Eldredge Bermingham, Jose Rovira, Oris Sanjur, AND Jan E. Conn*
Department of Natural Resource Sciences and the Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada; Smithsonian Tropical Research Institute, Balboa Ancon, Panama; Instituto Conmemorativo Gorgas de Estudios de la Salud, Panama City, Panama; Griffin Laboratory, The Wadsworth Center, New York State Department of Health, Slingerlands, New York

 

ABSTRACT

We report Anopheles darlingi in Darien Province in eastern Panama. Polymerase chain reaction–restriction fragment length polymorphism profiles of the single copy nuclear white gene and sequence comparisons confirmed the presence of 66 specimens of the northern lineage of An. darlingi. The parsimony network depicted 5 CO1 haplotypes in 40 specimens of An. darlingi, which connected through 7–8 mutational steps with sequences from Central and South America. Furthermore, the presence of haplotypes in Biroquera, Darien Province identical to those previously published from northern Colombia suggests that Panamanian samples originated in Colombia. Results of neutrality tests (R2 and Fu’s FS) were not significant and the mismatch distribution was multimodal and did not fit the model of sudden population growth. These findings may indicate a long and stable presence of An. darlingi in eastern Panama.

Received November 13, 2008. Accepted for publication February 11, 2009.

Acknowledgments: We thank Jorge Motta (Director, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panama City, Panama) for logistical support; Lisa Mirabello (Division of Cancer Epidemiology and Genetics, National Institutes of Health Bethesda, MD) for laboratory assistance; Urbano Arrocha and Silvio Bethan-court (Departamento de Control de Vectores del Ministerio de Salud, Panama City, Panama) and Wesley Harlow (The Wadsworth Center, New York State Department of Health) for fieldwork and local collaborations; and Sara Bickersmith (Griffin Laboratory, New York State Department of Health), Maribel Gonzales, Larissa Dutari, and Grethel Grajales (Smithsonian Tropical Research Institute) for technical input and suggestions for organizing the field trip.

Financial support: This study was supported by the Secretariat for Science, Technology and Innovation of Panama through research grant COL08-066 to Jose Loaiza, the Smithsonian Tropical Research Institute, the Institute of Parasitology of McGill University through its Centre for Host-Parasite Interactions travel fellowship awards program, and National Institutes of Health grant AI R0154139 to Jan E. Conn.

* Address correspondence to Jan E. Conn, Griffin Laboratory, The Wadsworth Center, New York State Department of Health, Slingerlands, NY 12159. E-mail: jconn{at}wadsworth.org

Authors’ addresses: Jose Loaiza, Department of Natural Resource Sciences, McGill University, 21,111 Lakeshore Road, Ste. Anne de Bellevue, Quebec H9X 3V9, Canada, E-mail: jose.loaiza{at}mail.mcgill.ca. Marilyn Scott, Institute of Parasitology, McGill University, Anne de Bellevue, Quebec H9X 3V9, Canada, E-mail: marilyn.scott{at}mcgill.ca. Eldredge Bermingham and Oris Sanjur, Smithsonian Tropical Research Institute, Apartado postal 0843-03092, Unit 0948, Balboa Ancon, Panama, E-mail: bermingham{at}si.edu. Jose Rovira, Departamento de Entomologia Medica, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panama City, Panama. Jan E. Conn, Griffin Laboratory, The Wadsworth Center, New York State Department of Health, Slingerlands, NY 12159, E-mail: jconn{at}wadsworth.org.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2009 by the American Society of Tropical Medicine and Hygiene.