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Am. J. Trop. Med. Hyg., 81(1), 2009, pp. 156-162
Copyright © 2009 by The American Society of Tropical Medicine and Hygiene

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MM3-ELISA Detection of Fasciola hepatica Coproantigens in Preserved Human Stool Samples

Florencio M. Ubeira*, Laura Muiño, M. Adela Valero, M. Victoria Periago, Ignacio Pérez-Crespo, Mercedes Mezo, Marta González-Warleta, Fernanda Romarís, Esperanza Paniagua, Sandra Cortizo, José Llovo, AND Santiago Más-Coma
Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, Santiago de Compostela, Spain; Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain; Centro de Investigaciones Agrarias, Mabegondo, A Coruña, Spain; Servicio de Microbiología, Complejo Hospitalario Universitario de Santiago (CHUS), Santiago de Compostela, Spain

In this study, we evaluate the MM3-COPRO method for detection of Fasciola coproantigens in human fecal samples, and the usefulness of a new preservative/diluent, CoproGuard, developed for preservation of Fasciola coproantigens. The MM3-COPRO assay was evaluated with 213 samples from healthy patients, 30 Fasciola positive fecal samples (according to the Kato-Katz method), and 83 samples from patients with other parasitic infections. All Fasciola positive specimens were detected with the MM3-COPRO assay (100% sensitivity) and there was no cross-reactivity with other common parasites present in the clinical specimens analyzed (100% specificity). The use of CoproGuard enhanced coproantigen extraction without affecting the detection limit of the assay, and the antigenicity of Fasciola coproantigens in fecal samples stored at 37°C was retained throughout the entire observation period (120 days). We concluded that the MM3-COPRO ELISA combined with the use of CoproGuard may be a very useful tool for the diagnosis of human fascioliasis.

Received January 19, 2009. Accepted for publication March 16, 2009.

Financial support: This study was supported by grant PGIDIT05 RAG20301PR from the Consellería de Innovación e Industria (Xunta de Galicia, Spain), by grants FAU2006-00021-C03-00, AGL-2006-13936-C02-01, and SAF2006-09278 from the Spanish Ministerio de Ciencia y Tecnología, and by the Red de Investigación de Centros de Enfermedades Tropicales–RICET (Projects PI030545 and RD06/0021/0017 of the Programme of Redes Temáticas de Investigación Cooperativa of the Fondo de Investigación Sanitaria (FIS), Ministerio de Sanidad, Spain.

* Address correspondence to Florencio M. Ubeira, Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain. E-mail: fm.ubeira{at}usc.es

Authors’ addresses: Florencio M. Ubeira, Laura Muiño, Fernanda Romarís, and Esperanza Paniagua, Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain. M. Adela Valero, M. Victoria Periago, Ignacio Pérez-Crespo, and Santiago Más-Coma, Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain. Mercedes Mezo and Marta González-Warleta, Centro de Investigaciones Agrarias, Mabegondo, PO Box 10, 15080, A Coruña, Spain. Sandra Cortizo and José Llovo, Servicio de Microbiología, Complejo Hospitalario Universitario de Santiago (CHUS), Santiago de Compostela, Spain.






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