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Increased Phagocytosis of Platelets from Patients with Secondary Dengue Virus Infection by Human Macrophages

Shoko Honda, Mariko Saito, Efren M. Dimaano, Philip A. Morales, Maria T. G. Alonzo, Lady-Anne C. Suarez, Natsuki Koike, Shingo Inoue, Atsushi Kumatori, Ronald R. Matias, Filipinas F. Natividad, AND Kazunori Oishi^*

The relationship between the percent phagocytosis of platelets by differentiated THP-1 cells was examined using flowcytometry and the peripheral platelet counts as well as platelet-associated IgG (PAIgG) in 36 patients with secondary dengue virus (DV) infections. The percent phagocytosis and the levels of PAIgG were significantly increased in these patients during the acute phase compared with the healthy volunteers. The increased percent phagocytosis and PAIgG found during the acute phase significantly decreased during the convalescent phase. An inverse correlation between platelet count and the percent phagocytosis (P = 0.011) and the levels of PAIgG (P = 0.041) was found among these patients during the acute phase. No correlation was found, however, between the percent phagocytosis and the levels of PAIgG. Our present data suggest that accelerated platelet phagocytosis occurs during the acute phase of secondary DV infections, and it is one of the mechanisms of thrombocytopenia in this disease.

Received July 9, 2008. Accepted for publication January 16, 2009.

Acknowledgments: We thank the staff of San Lazaro Hospital and the Research Biotechnology Division, St. Luke’s Medical Center.

Financial support: This study was supported by a Grant-in-Aid for Scientific Research (B: 16406029) from the Ministry of Education, Science and Culture, Japan and the 21st Century Center of Excellence (COE) Program of Nagasaki University.

^* Address correspondence to Kazunori Oishi, Laboratory for Clinical Research on Infectious Diseases, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, 3-1, Yamadaoka, Japan. E-mail: oishik{at}biken.osaka-u.ac.jp

Authors’ addresses: Shoko Honda and Shingo Inoue, Department of Internal Medicine and Virology, Institute of Tropical Medicine Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan. Mariko Saito, Department of Virology, Graduate School of Medicine, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan. Efren M. Dimaano and Philip A. Morales, Blood Borne Diseases, San Lazaro Hospital, Manila, Philippines. Maria T. G. Alonzo, Ronald R. Matias, and Filipinas F. Natividad, Research and Biotechnology Division, St. Luke’s Medical Center, 279 E. Rodriguez Sr. Boulevard, Cathedral Heights, Quezon City, Philippines 1102. Lady-Anne C. Suarez, E-mail: suarezlacs{at}yahoo.com. Natsuki Koike, E-mail: nakkey2{at}yahoo.co.jp. Atsushi Kumatori, Faculty of Risk and Crisis Management, Chiba Institute of Science, Choshi 288-0025, Japan. Kazunori Oishi, Laboratory for Clinical Research on Infectious Diseases, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan, Tel: +81-6-6879-4253, Fax: +81-6-6879-4255, E-mail: oishik{at}biken.osaka-u.ac.jp.

S. Honda and M. Saito equally contributed to the work described in this article.

Reprint requests: Kazunori Oishi, Laboratory for Clinical Research on Infectious Diseases, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Osaka 565-0871, Japan, Tel: +81-6-6879-4253, Fax: +81-6-6879-4255, E-mail: oishik{at}biken.osaka-u.ac.jp.