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Am. J. Trop. Med. Hyg., 80(5), 2009, pp. 798-804
Copyright © 2009 by The American Society of Tropical Medicine and Hygiene

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Morphologic Organization of Pulmonary Granulomas in Mice Infected with Paracoccidioides brasiliensis

Fernanda C. Da Silva, Terezinha I. E. Svidzinski, Eliana V. Patussi, Cristina P. Cardoso, Márcia M. De Oliveira Dalalio, AND Luzmarina Hernandes*
Post-graduate Program in Clinical Analyses, Laboratory of Medical Mycology, Laboratory of Immunology, Department of Clinical Analyses, Laboratory of Histology, Department of Morphophysiological Sciences, Universidade Estadual de Maringá, Maringá, Paraná, Brazil

A morphologic study of the lungs was carried out in Swiss mice infected with yeast isolated from Paracoccidioides brasiliensis (Pb18). The lung was processed 1, 2, 4, and 8 weeks after inoculation for histologic staining with hematoxylin and eosin (H&E), methenamine silver nitrate (Gomori–Grocott), and picrosirius to qualitative and quantitative analyses of the granulomas and the presence of fungal lesions. The numbers of CFUs/g counted in the lungs were 189.8 ± 20.64, 353.6 ± 46.21, 547.2 ± 108.1, and 295.2 ± 89.17 in the first, second, fourth, and eighth weeks, respectively. One week after infection, inflammatory cells and reticular and collagens fibers, the latest typical of fibrosis, were detected. After 2 and 4 weeks, a progressive intensification of the infection and fibrosis was observed, but in week 8 a more organized granuloma was evident, with macrophages, epithelioid cells, and yeasts in the central portion, and intense peripheral basophilia. Pycnotic structures typical of apoptotic bodies were observed in weeks 1 and 8. The different histologic staining used acted as a fundamental tool for the study of the morphologic organization of granuloma formation.

Received March 19, 2008. Accepted for publication November 26, 2008.

Acknowledgments: We thank UEM/CNPq for financial support for this research, and Maria Euride Carlo Cancino, Maria dos Anjos Fortunato, Ana Paula Santi, and Tânia Pereira Salci for technical support. The manuscript was translated by Peter Grimshaw. The American Society of Tropical Medicine and Hygiene (ASTMH) assisted with publication expenses.

* Address correspondence to Luzmarina Hernandes, Laboratory of Histology, Departamento de Ciências Morfofisiológicas, Universidade Estadual de Maringá, Avenida Colombo 5790, cep 87020 900, Maringá, Paraná, Brazil. E-mail: lhernandes{at}uem.br

Authors’ addresses: Fernanda C. Da Silva and Luzmarina Hernandes, Laboratory of Histology, Departamento de Ciências Morfofisiológicas, Universidade Estadual de Maringá, Avenida Colombo 5790, cep 87020 900, Maringá, Paraná, Brazil, E-mails: lhernandes{at}uem.br and nandacristie{at}gmail.com. Terezinha I. E. Svidzinski and Eliana V. Patussi, Laboratory of Medical Mycology, Universidade Estadual de Maringá, Avenida Colombo 5790, cep 87020 900, Maringá, Paraná, Brazil, E-mails: tiesvidzinski{at}uem.br and evpatussi{at}gmail.com. Cristina P. Cardoso and Márcia M. De Oliveira Dalalio, Laboratory of Immunology, Departamento de Análises Clínicas. Universidade Estadual de Maringá, Avenida Colombo 5790, cep 87020 900, Maringá, Paraná, Brazil, E-mails: crispadre{at}yahoo.com.br and mmodalalio{at}uem.br.






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