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Am. J. Trop. Med. Hyg., 80(4), 2009, pp. 625-627
Copyright © 2009 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


Comparison of Culture Techniques at Different Stages of Brucellosis

Benjamin J. Espinosa*, Jesús Chacaltana, Maximilian Mulder, María Pía Franco, David L. Blazes, Robert H. Gilman, Henk L. Smits, AND Eric R. Hall
Naval Medical Research Center Detachment, Lima, Peru; Hospital Nacional Daniel Alcides Carrion, Callao, Peru; A. B. Prisma, Lima, Peru; Department of Internal Medicine, Hennepin County Medical Center, Minneapolis, Minnesota; Department of Neurology, University of Minnesota, Minneapolis, Minnesota; Royal Tropical Institute, Amsterdam, The Netherlands

 

ABSTRACT

The lysis centrifugation technique is preferred for culturing Brucella spp. at all stages of brucellosis because it yields 25% more positive results and on average provides results 10 days earlier than the Ruiz-Castaneda method. This lysis method is inexpensive and easier to use and may be used in laboratories with limited expertise or equipment if all safety precautions are taken.



Received May 2, 2008. Accepted for publication December 20, 2008.

Disclaimer: The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, or the U.S. government. Drs. Benjamin J. Espinosa, David L. Blazes, and Eric R. Hall are U.S. military service members working at the U.S. Navy Medical Research Center Detachment in Lima, Peru. This work was prepared as part of their official duties. Title 17 U.S.C. § 105 provides that Copyright protection under this title is not available for any work of the United States Government. Title 17 U.S.C. § 101 defines a U.S. Government work as a work prepared by a military service member or employee of the U.S. Government as part of that person’s official duties.

* Address correspondence to Benjamin J. Espinosa, Naval Medical Research Center Detachment, Lima, Peru. E-mail: benjamin.espinosa{at}med.navy.mil

Authors’ addresses: Benjamin J. Espinosa, David L. Blazes, and Eric R. Hall, Naval Medical Research Center Detachment, Lima, Peru. Jesús Chacaltana, Hospital Nacional Daniel Alcides Carrión, Callao, Peru. Maximilian Mulder, Department of Internal Medicine, Hennepin County Medical Center, Minneapolis, MN 55415. María Pía Franco, Department of Neurology, University of Minnesota, Minneapolis, MN 55415. Robert H. Gilman, Department of International Health, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Room W5515, Baltimore, MD 21205. Henk L. Smits, Royal Tropical Institute, Amsterdam, The Netherlands.




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J. Clin. Microbiol.Home page
K. Nockler, R. Maves, D. Cepeda, A. Draeger, A. Mayer-Scholl, J. Chacaltana, M. Castaneda, B. Espinosa, R. Castillo, E. Hall, et al.
Molecular Epidemiology of Brucella Genotypes in Patients at a Major Hospital in Central Peru
J. Clin. Microbiol., October 1, 2009; 47(10): 3147 - 3155.
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