AJTMH HINARI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 80(4), 2009, pp. 593-595
Copyright © 2009 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pita-Pereira, D.
Right arrow Articles by Rangel, E. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pita-Pereira, D.
Right arrow Articles by Rangel, E. F.

SHORT REPORT


First Report of Lutzomyia (Nyssomyia) neivai (Diptera: Psychodidae: Phlebotominae) Naturally Infected by Leishmania (Viannia) braziliensis in a Periurban Area of South Brazil Using a Multiplex Polymerase Chain Reaction Assay

Daniela Pita-Pereira, Getúlio D. Souza, Adriana Zwetsch, Carlos Roberto Alves, Constança Britto, AND Elizabeth F. Rangel*
Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brasil; Seção de Reservatórios e Vetores, Instituto de Pesquisas Biológicas, Laboratório Central de Pública do Rio Grande do Sul, Fundação Estadual de Produção e Pesquisa em Saúde, Secretaria Estadual de Saúde do Rio Grande do Sul; Núcleo de Vigilância de Roedores e Vetores, Coordenadoria Geral de Vigilância em Saúde, Secretaria Municipal de Saúde de Porto Alegre; Laboratório de Transmissores de Leishmanioses, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brasil

 

ABSTRACT

To identify Lutzomyia (Nyssomyia) neivai naturally infected by Leishmania a multiplex polymerase chain reaction (PCR) was used for the analysis of 450 specimens (270 females, 180 males) collected in an endemic periurban area of cutaneous leishmaniasis in Porto Alegre, Brazil. Insects were grouped into pools of 10 and positive results were achieved in 3/27 Lu. (N.) neivai female pools. Infection by L. (Viannia) braziliensis was confirmed after hybridizing PCR products with a subgenus–specific biotinylated probe. Considering the detection of three positive pools with at least one infected insect in each, an infection rate of 1.1% was estimated. Our results associated with epidemiologic data suggest a potential ability of Lu. (N.) neivai in transmitting L. braziliensis in Porto Alegre, where the first notifications of autochthonous cutaneous leishmaniasis in humans occurred in 2002, with an increase in the number of cases in recent years possibly as a consequence of deforestation and agricultural activities in the area.

Received August 22, 2008. Accepted for publication December 10, 2008.

Acknowledgments: We thank J. A. Ferreira, A. N. Martínez, and B. S. Pereira for the English revision.

Financial support: This investigation was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico–CNPq, Brasil (476380/2004-9).

Disclosure: Daniela Pita-Pereira is a fellow PhD student from FIOCRUZ/CAPES, Carlos R. Alves, Constança Britto, and Elizabeth F. Rangel are fellows of the CNPq Institution, and Adriana Zwetsch is a fellow of the FAPERJ Institution.

* Address correspondence to Elizabeth Rangel, Laboratório de Transmissores de Leishmanioses, Instituto Oswaldo Cruz, FIOCRUZ, Pavilhão Carlos Chagas, Avenida Brasil 4365, Manguinhos, 21045-900, Rio de Janeiro, RJ, Brasil. E-mail: elizabethrangel{at}fiocruz.br

Authors’ addresses: Daniela Pita-Pereira, Carlos R. Alves, and Constança Britto, Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, FIOCRUZ, Pavilhão Leônidas Deane sala 209, Avenida Brasil 4365, Manguinhos, 21045-900, Rio de Janeiro, RJ, Brasil. Getúlio D. Souza, Seção de Reservatórios e Vetores, Instituto de Pesquisas Biológicas, Laboratório Central de Pública do Rio Grande do Sul, Fundação Estadual de Produção e Pesquisa em Saúde, Secretaria Estadual de Saúde do Rio Grande do Sul, Avenida Ipiranga 5400, Bairro Jardim Botânico, 90610-000, Porto Alegre, RS, Brasil; Núcleo de Vigilância de Roedores e Vetores, Coordenadoria Geral de Vigilância em Saúde, Secretaria Municipal de Saúde de Porto Alegre, Avenida Padre Cacique 372, Bairro Menino Deus, 90810-240, Porto Alegre, RS, Brasil. Adriana Zwetsch and Elizabeth F. Rangel, Laboratório de Transmissores de Leishmanioses, Instituto Oswaldo Cruz, FIOCRUZ, Pavilhão Carlos Chagas, Avenida Brasil 4365, Manguinhos, 21045-900, Rio de Janeiro, RJ, Brasil, Tel: +55-21-2590-9590, Fax: +55-21-2560-7011, E-mail: elizabethrangel{at}fiocruz.br.

Reprint requests: Elizabeth Rangel, Laboratório de Transmissores de Leishmanioses, Instituto Oswaldo Cruz, FIOCRUZ, Pavilhão Carlos Chagas, Avenida Brasil 4365, Manguinhos, 21045-900, Rio de Janeiro, RJ, Brasil.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2009 by the American Society of Tropical Medicine and Hygiene.