AJTMH HINARI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 80(3), 2009, pp. 339-344
Copyright © 2009 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Matta, N. E.
Right arrow Articles by Da-Cruz, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matta, N. E.
Right arrow Articles by Da-Cruz, A. M.

Leishmania (Viannia) guyanensis Induces Low Immunologic Responsiveness in Leishmaniasis Patients from an Endemic Area of the Brazilian Amazon Highland

Nubia Estela Matta, Ricardo S. Nogueira, Antonia Maria Ramos Franco, Ilner de Souza e Souza, Marise S. Mattos, Manoel P. Oliveira-Neto, Sergio G. Coutinho, Leonor L. Leon, AND Alda Maria Da-Cruz*
Laboratório de Imunoparasitologia, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil; Laboratório de Bioquímica de Tripanosomatídeos, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil; Hospital Alfredo da Matta, Manaus, Brazil; Coordenação de Pesquisas em Ciências da Saúde, Instituto Nacional de Pesquisa da Amazônia-INPA, Manaus, Brazil; Instituto de Pesquisa Clínica Evandro Chagas (IPEC), FIOCRUZ, Rio de Janeiro, Brazil; Departamento de Biología, Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá, Colombia

Cutaneous leishmaniasis caused by Leishmania (Viannia) guyanensis (CL-Lguy) is endemic in the Brazilian Amazon, differing from L. braziliensis infection in clinical, diagnostic, and therapeutic aspects. T-cell reactivity to leishmanial antigens possibly involved in the pathogenesis of CL-Lguy was studied herein. Variable lymphoproliferative responses (LPRs) to Leishmania antigens were found among the 23 studied patients, and 50% of them showed low or no response to these antigens. Active disease was associated with an enrichment of leishmanial-reactive T lymphocytes, mainly TCD4+. High and low interferon (IFN)-{gamma} producers were observed. TNF-{alpha}, interleukin (IL)-10, and IL-5 were consistently detected. CL-Lguy displayed low antibody response in comparison to L. braziliensis patients. CL caused by L. braziliensis presented positive LPRs and higher IFN-{gamma} production but undetectable IL-5. L. guyanensis seems to induce a down-regulation of the immune system compared with L. braziliensis. This finding could explain some aspects of clinical presentation of CL-Lguy, such as high tissue parasite burden and frequent resistance to therapy.

Received February 11, 2008. Accepted for publication May 20, 2008.

Acknowledgments: We thank Dr. Yara Traub Cseko and Dr. Octávio Fernandes for valuable comments and English review, Dr. Alvaro Bertho for flow cytometry analysis, Dr. Wilson J. S. Souza for immunofluorescence assays, Luanda Figueira, MSc, Rita Bittar, BSc, Gerzia Machado, MSc, and Adriano G. Silva for help and generous assistance in the laboratory, and Rosangela Pellegrino for secretarial assistance. We also thank Dr. Elisa Cupolillo at the Coleção de Leishmania do Instituto Oswaldo Cruz (CLIOC WFCC WDCM731), Fundação Oswaldo Cruz for identification of parasites.

Financial support: This work was supported by FAPERJ (Grant E-26/170.844/2003), IOC/FIOCRUZ internal funds, PAPES/VPPDT/FIOCRUZ, RENOR/CAPES, and INPA. NEM was sponsored by CNPq, Brazil. AMC and LL are CNPq research fellows.

Disclosure: There are no commercial or other associations that might pose a conflict of interest.

* Address correspondence to Alda Maria Da-Cruz, Laboratório de Imunoparasitologia, Instituto Oswaldo Cruz-FIOCRUZ, Av. Brasil 4365, Pav. Leônidas Deane, 4°. andar, Manguinhos, Rio de Janeiro-RJ CEP 21040-900, Brazil. E-mail: alda{at}ioc.fiocruz.br

Authors’ addresses: Nubia Estela Matta, Ricardo S. Nogueira, Sergio G. Coutinho, and Alda Maria Da-Cruz, Laboratório de Imunoparasitologia/IOC-FIOCRUZ. Av. Brasil 4365 Pav. Leonidas Deane 4°. andar. Manguinhos, Rio de Janeiro, RJ, Brazil, CEP 21040-900, Tel: 00-55-21-3865-8174/8102, Fax: 00-55-21- 2290-0479. Antonia Maria Ramos Franco, Instituto Nacional de Pesquisas da Amazônia, Departamento de Ciências da Saúde, Divisão de Parasitologia I, Laboratório de Leishmanioses e Doença de Chagas, Av. André Araújo, 1756–Aleixo. CEP 69083-000 Manaus, AM, Brazil, Tel: 92-6433065, Fax: 92-6433061. Ilner de Souza e Souza, Faculdade de Ciências da Saúde/UFAM, Departamento de Saúde Coletiva. Rua Afonso Pena, 1053, Praça 14 Manaus, AM, Brazil. Marise S. Mattos and Manoel P. Oliveira-Neto, Instituto de Pesquisa Clínica Evandro Chagas IPEC/FIOCRUZ. Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ, Brazil, CEP 21040-900. Leonor L. Leon, Laboratório de Bioquímica de Tripanossomatídeos/IOC-FIOCRUZ. Av. Brasil 4365, Pav. Leonidas Deane 4°. andar. Manguinhos, Rio de Janeiro, RJ, Brazil, CEP 21040-900, Tel: 00-55-21-3865-8174/8102, Fax: 00-55-21-2290-0479.

Reprint requests: Alda Maria Da-Cruz, Laboratório de Imunoparasitologia/IOC-FIOCRUZ. Av. Brasil 4365 Pav. Leonidas Deane 4°. andar, Manguinhos, Rio de Janeiro, RJ, Brazil, CEP 21040-900, E-mail: alda{at}ioc.fiocruz.br.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2009 by the American Society of Tropical Medicine and Hygiene.