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Am. J. Trop. Med. Hyg., 80(2), 2009, pp. 239-241
Copyright © 2009 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


Concurrent Detection of Trypanosoma cruzi Lineages I and II in Domestic Triatoma dimidiata from Guatemala

Pamela M. Pennington*, Claudia Paiz, Laura M. Grajeda, AND Celia Cordón-Rosales
Center for Health Studies, Universidad del Valle de Guatemala, Guatemala City, Guatemala

 

ABSTRACT

The agent of Chagas disease, Trypanosoma cruzi, is phylogenetically divided into two lineages, T. cruzi I (TCI) and II (TCII). TCI is found in sylvatic and domestic habitats across South America. Despite a high prevalence of TCII in domestic habitats in South America, it has been rarely found in domestic habitats in Central America and Mexico. This may be caused by limitations in detection tests previously used. A modified hemi-nested polymerase chain reaction assay was developed to improve detection of TCI and TCII mini-exon genes. This method detected TCI and II concurrently in 84% of 44 cultured isolates from Triatoma dimidiata specimens collected inside homes across the disease-endemic area in Guatemala.

Received July 8, 2008. Accepted for publication October 20, 2008.

Acknowledgments: We thank Ben Beard and Ellen Dotson for their support during these research projects, and Sandra Juarez, Ben Beard, and Henry Bishop for providing control strains.

Financial support: This study was partially supported by grants from the Gorgas Memorial Research Institute Award and Tropical Disease Research grant A30460 to Pamela M. Pennington, and project 085–2006, "Estudio del Ciclo de Transmisión del Trypanosoma cruzi Mediante la Tipificación Genética de Este Parásito," funded by Fondo para el Desarrollo de la Ciencia y Tecnología within Consejo Nacional de Ciencia y Tecnología to Laura M. Grajeda.

* Address correspondence to Pamela M. Pennington, Center for Health Studies, Research Institute, Universidad del Valle de Guatemala, 18 Avenida 11-95 Zona 15 VHIII, Guatemala City, Guatemala, 01015. E-mail: pamelap{at}uvg.edu.gt

Authors’ addresses: Pamela M. Pennington, Claudia Paiz, Laura M. Grajeda, and Celia Cordón-Rosales, Center for Health Studies, Research Institute, Universidad del Valle de Guatemala, 18 Avenida 11-95 Zona 15 VHIII, Guatemala City, Guatemala, 01015, E-mails: pamelap{at}uvg.edu.gt and ccordon{at}gt.cdc.gov.






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Copyright © 2009 by the American Society of Tropical Medicine and Hygiene.