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Am. J. Trop. Med. Hyg., 80(1), 2009, pp. 55-60
Copyright © 2009 by The American Society of Tropical Medicine and Hygiene

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Enzyme-linked Immunosorbent Assay for Superoxide Dismutase–Excreted Antigen in Diagnosis of Sylvatic and Andean Cutaneous Leishmaniasis of Peru

Clotilde Marín, Silvia S. Longoni, Jesús Urbano, Gloria Minaya, Hector Mateo, Jose A. de Diego, María José Rosales, Gregorio Pérez-Cordón, Desiré Romero, AND Manuel Sánchez-Moreno*
Instituto de Biotecnología, Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Granada, Spain; Laboratorio de Leishmaniosis y Chagas, Instituto Nacional de Salud, Lima, Perú; Departamento de Estadística e Investigación Operativa, Facultad de Ciencias, Universidad de Granada, Granada, Spain; Unidad de Parasitología y Medicina Tropical, Departamento de Medicina Preventiva y Salud Pública, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain

A superoxide dismutase excreted by promastigote forms of L. (Viannia) peruviana (SODe-Lp), L. (Viannia) brazilensis (SODe-Lb), and L. (L.) amazonensis (SODe-La) is tested to evaluate its potential value as a diagnostic tool of mucocutaneous and Andean cutaneous leishmaniasis. We used 45 sera with mucocutaneous leishmaniasis (SL) and 68 with Andean cutaneous leishmaniasis (ACL). SODe-Lp antigen was recognized by 94% of the serum from ACL patients, and the SODe-Lb antigen was recognized by 93% of the serum from SL patients. Meanwhile, the result for SL and ACL patients with SODe-La antigen was 69% and 43% and SODe-Li was 11% and 9%, respectively. This suggest that antibodies to SODe-Lp undergo further response in patients with ACL and the antibodies to SODe-Lb do so preferentially in patients with SL. The SODe ELISA may be useful in endemic areas for discriminative assays between patients with different forms of leishmaniases and those with other clinical conditions.

Received December 21, 2007. Accepted for publication August 13, 2008.

Financial support: This work was supported by the CGL2006-27889-E/BOS: Biobank and Unity of Characterization of trypanosoma-tid species and strains responsible of human, animal and vegetable pathologies.

* Address correspondence to Manuel Sánchez-Moreno, Departamento de Parasitología, Facultad de Ciencias, Instituto de Biotecnología, Universidad de Granada, Granada 18071, Spain. E-mail: msanchem{at}ugr.es

Authors’ addresses: Clotilde Marín, Silvia S. Longoni, Jesús Urbano, Hector Mateo, María José Rosales, Gregorio Pérez-Cordón, and Manuel Sánchez-Moreno, Instituto de Biotecnología, Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Granada, Spain. Gloria Minaya, Laboratorio de Leishmaniosis y Chagas, Instituto Nacional de Salud, Lima, Perú. José A. De Diego, Unidad de Parasitología y Medicina Tropical, Departamento de Medicina Preventiva y Salud Pública Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain. Desiré Romero, Departamento de Estadística e Investigación Operativa, Facultad de Ciencias, Universidad de Granada, Granada, Spain.






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Copyright © 2009 by the American Society of Tropical Medicine and Hygiene.