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Isolation of a Type 2 Dengue Virus by Use of Hamster Kidney Cell Cultures^*

Fred H. Diercks

Attempts to recover dengue virus from 5 acute phase human serum specimens which had been stored at CO₂ icebox temperatures for a period of 12 years were carried out in hamster kidney cell cultures. The one successful isolation attempt yielded a type 2 dengue fever virus. The importance of the procedures used for increasing the probability of recovery of viruses from virus-containing materials was discussed.

^* A portion of a dissertation submitted to the Graduate School of Public Health, University of Pittsburgh, in partial satisfaction of the requirements for the degree of Doctor of Science in Hygiene. This work was done under sponsorship of the Commission on Viral Infections of the Armed Forces Epidemiological Board, and supported in part by the Office of the Surgeon General, Department of the Army.

Major, MSC, now stationed at Walter Reed Army Institute of Research, Washington, D. C.