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Am. J. Trop. Med. Hyg., 79(6), 2008, pp. 951-954
Copyright © 2008 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


Comparison of Oral Infectious Dose of West Nile Virus Isolates Representing Three Distinct Genotypes in Culex quinquefasciatus

Dana L. Vanlandingham, Charles E. McGee, Kimberly A. Klingler, Sareen E. Galbraith, Alan D. T. Barrett, AND Stephen Higgs*
Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas

 

ABSTRACT

Phylogenetic analysis of West Nile virus in North America has identified replacement of the originally introduced clade, Eastern United States (NY99), by the North American clade. In addition, the transient emergence of other clades and genetic variants has also been observed. In this study, we investigated the potential role of the mosquito in the selection of these clades and genetic variants. We determined the relative susceptibility of Culex quinquefasciatus to infection with isolates from the Eastern U.S. clade, the North American clade, and the Southeast coastal Texas clade. Although significant differences were observed in 50% oral infectious dose values between the Eastern U.S. and two attenuated North American genetic variants compared with the North American and Southeast coastal Texas clade viruses, these differences did not correlate with persistence of the genotype in nature. These results indicate that selection of these viral genotypes was independent of viral oral infectivity in the mosquito.

Received February 20, 2008. Accepted for publication July 12, 2008.

Acknowledgment: We thank Jing Haung for expert technical assistance with this project.

Financial support: This study was supported in part by Centers for Disease Control and Prevention grant U50/CCU620539 to Stephen Higgs and National Institutes of Health (NIH) grant RO1 AI 67847 to Alan D. T. Barrett. Dana L. Vanlandingham was supported in part by NIH grant T32 A107536. Charles E. McGee was supported by the Centers for Disease Control and Prevention Fellowship Training Program in Vector-Borne Infectious Diseases (TOI/CCT622892) and NIH grant T32 AI 7526.

* Address correspondence to Stephen Higgs, Department of Pathology, University of Texas Medical Branch, Keiller Building, Room 2.104, 301 University Boulevard, Galveston, TX 77555-0609. E-mail: sthiggs{at}utmb.edu

Authors’ address: Dana L. Vanlandingham, Charles E. McGee, Kim-berly A. Klingler, Sareen E. Galbraith, Alan D. T. Barrett, and Stephen Higgs, Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Keiller Building, Room 2.104, 301 University Boulevard, Galveston, TX 77555-0609, Tel: 409-772-2511, Fax: 409-747-2437, E-mail: sthiggs{at}utmb.edu.






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Copyright © 2008 by the American Society of Tropical Medicine and Hygiene.