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Serologic Survey of the Sentinel Animals for Plague Surveillance and Screening for Complementary Diagnostic Markers to F1 Antigen by Protein Microarray

Bei Li, Ying Guo, Zhaobiao Guo, Yun Liang, Ziwen Zhu, Qing Zhou, Yanfeng Yan, Zhizhong Song^*, AND Ruifu Yang^*

Plague is a deadly infectious disease caused by the gram-negative bacterium, Yersinia pestis. In 2005, five plague patients were confirmed in the Yulong County of the Yunnan Province, China. In this study, the serologic survey of > 2,900 serum samples from domestic dogs and cats in and around the county, where human plague occurred, confirmed that domestic dogs and cats could serve as sentinel animals for plague surveillance. Meanwhile, the antibody responses in the infected dogs and cats were profiled by microarray containing 218 proteins of Y. pestis. In addition to F1, LcrV, YPCD1.28c, and YPO2118 induced humoral responses in all or most of the individuals, providing complementary candidates to F1 antigen for diagnostic markers of plague.

Financial support: This work was supported by the National 863 Project (Contract 2006AA02Z438), the National Natural Science Foundation of China (Grants 30430620 and 30771920), and the Social Development Foundation of Yunnan Province (Grant 2008CA010).

^* Address correspondence to Ruifu Yang, Professor No. 20, Dongdajie, Fengtai, Beijing 100071, China, E-mail: ruifuyang{at}gmail.com and Zhizhong Song, Professor No. 5, Wenhualu, Dali 671000, Yunnan Province, China, E-mail: Song1208{at}126.com

These authors contributed equally to this work.

Authors’ addresses: Bei Li, Zhaobiao Guo, Ziwen Zhu, Yanfeng Yan, and Ruifu Yang, No. 20, Dongdajie, Fengtai, Beijing 100071, China, Tel: 0086-10-66948595, Fax: 0086-10-63815689. Ying Guo, Yun Liang, Qing Zhou, and Zhizhong Song, No. 5, Wenhualu, Dali 671000, Yunnan Province, China, Tel: 0086-872-2192058, Fax: 0086-872-2125437.

Note: Supplementary Table S1 (Y. pestis proteins represented on the microarray), Supplementary Table S2 (The original data of antibody fluorescent value against the protein on the Microarray), Supplementary Figure S1 (The geographic distribution of the seropositive rate in the satellite map of Yulong and around County) and Supplementary Figure S2 (Antibody profile by the protein microarray) appear online at www.ajtmh.org.