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Am. J. Trop. Med. Hyg., 79(5), 2008, pp. 706-707
Copyright © 2008 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


First Molecular Identification of Entamoeba moshkovskii in Human Stool Samples in Tunisia

Soumaya Ben Ayed, Karim Aoun, Nadia Maamouri, Rym Ben Abdallah, AND Aïda Bouratbine*
Laboratoire de recherche 05SP03, Laboratoire de Parasitologie, Institut Pasteur de Tunis, Tunis, Tunisia; Service de Gastroentérologie La Rabta, Tunis, Tunisia

 

ABSTRACT

We report the first intestinal infections in Tunisia with Entamoeba moshkovskii in two healthy adults. Entamoeba moshkovskii cysts were distinguished from those of the morphologically identical parasites Entamoeba histolytica and Entamoeba dispar by specific nested polymerase chain reaction and sequencing.

Received April 28, 2008. Accepted for publication July 17, 2008.

Acknowledgments: We sincerely thank Dr. C. Graham Clark from London School of Hygiene and Tropical Medicine for providing us with lyophilized DNA of standard cultures of E. moshkovskii Laredo. We also thank Najet Zallaga from the Department of Parasitology, Pasteur Institute of Tunis for her cooperation and help.

Financial support: This study was supported by the Ministry of Higher Education, Research and Technology, Tunisia in the setting of the Reserch Lab "Parasitoses emergentes" LR 05SP03.

* Address correspondence to Aïda Bouratbine, LR "Parasitoses emergentes," Laboratoire de Parasitologie, Institut Pasteur de Tunis, 13 Place Pasteur, BP 74, 1002 Tunis Belvedere, Tunisia. E-mail: aida.bouratgine{at}pasteur.rns.tn

Authors’ addresses: Soumaya Ben Ayed, Karim Aoun, Rym Ben Abdallah and Aïda Bouratbine, LR "Parasitoses emergentes," Laboratoire de Parasitologie, Institut Pasteur de Tunis, 13 Place Pasteur, BP 74, 1002 Tunis belvedere, Tunisia. Nadia Maamouri, Service de Gastroentérologie La Rabta, 1007 Jebbari Tunis, Tunisia.






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Copyright © 2008 by the American Society of Tropical Medicine and Hygiene.