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Am. J. Trop. Med. Hyg., 79(5), 2008, pp. 685-687
Copyright © 2008 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


Dengue-3 Outbreak in Paraguay: Investigations Using Capillary Blood Samples on Filter Paper

Severine Matheus*, Jean-Baptiste Meynard, Anne Lavergne, Romain Girod, David Moua, Bhety Labeau, Philippe Dussart, Vincent Lacoste{dagger}, AND Xavier Deparis{dagger}
Centre National de Référence des Arbovirus, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’épidemiologie, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’entomologie Institut Pasteur de la Guyane, Cayenne, French Guiana; Laboratoire des Interactions Virus Hôtes, Institut Pasteur de la Guyane, Cayenne, French Guiana; Institut de Médecine Tropicale du Service de Santé des Armées, Le Pharo, Marseille, France

 

ABSTRACT

During a dengue-3 outbreak in Paraguay at the beginning of 2007, capillary blood samples absorbed onto filter papers were collected from 44 suspected cases. These samples were subjected to three molecular and serologic tests, and 31 of the 44 samples gave a positive result by at least one of the techniques used. Molecular analyses detected the dengue-3 serotype in 22 patients and additionally the dengue-2 serotype in two patients. Therefore two different serotypes were co-circulating during this outbreak. Overall, this study validates the use of dried-blood samples for field screening investigations. Indeed, all types of laboratory studies of dengue were possible with samples consisting of a few drops of dried blood from finger pricks.

Received April 4, 2008. Accepted for publication August 4, 2008.

Acknowledgments: We are grateful to Norma Coluchi of the Laboratorio Central de Salud Publica/Virology Department, and to Gualberto Pinanez, Maria Angelica Barbosa, and Cesia Feltes of the Ministerio de Salud Publica/Dirección General de Vigilancia de la Salud for their help in collecting data and samples in Paraguay.

Financial support: This work was supported by the Institut Pasteur de la Guyane.

* Address correspondence to Severine Matheus, Centre National de Référence des Arbovirus, Institut Pasteur de la Guyane, 23 Avenue Pasteur, BP 6010, 97306 Cayenne Cedex, French Guiana. E-mail: smatheus{at}pasteur-cayenne.fr

{dagger} Senior authorship: Vincent Lacoste and Xavier Deparis share senior authorship.

Authors’ addresses: Severine Matheus, David Moua, Bhety Labeau, and Philippe Dussart, Centre National de Référence des Arbo-virus, Institut Pasteur de la Guyane, 23 Avenue Pasteur, BP 6010, 97306 Cayenne Cedex, Tel: 05-94-29-58-16, Fax: 05-94-29-58-09, E-mails: smatheus{at}pasteur-cayenne.fr, dmoua{at}pasteur-cayenne.fr, blabeau{at}pasteur-cayenne.fr, and pdussart{at}pasteur-cayenne.fr. Jean-Baptiste Meynard, Unité d’Epidémiologie, Institut Pasteur de la Guyane, 23 Avenue Pasteur, BP 6010, 97306 Cayenne Cedex, Tel: 05-94-29-26-15, Fax: 05-94-29-58-09, E-mail: jbmeynard{at}pasteur-cayenne.fr. Anne Lavergne and Vincent Lacoste, Laboratoire des Interactions Virus Hôtes, Institut Pasteur de la Guyane, 23 Avenue Pasteur, BP 6010, 97306 Cayenne Cedex, Tel: 05-94-29-58-17, Fax: 05-94-29-58-26, E-mails: alavergne{at}pasteur-cayenne.fr and vlacoste{at}pasteur-cayenne.fr. Romain Girod, Unité d’Entomologie, Institut Pasteur de la Guyane, 23 Avenue Pasteur, BP 6010, 97306 Cayenne Cedex, Tel: 05-94-29-26-03, Fax: 05-94-29-58-09, E-mail: rgirod{at}pasteur-cayenne.fr. Xavier Deparis, Département d’Epidémiologie et de Santé Publique, Institut de Médecine Tropicale et de santé publique, Marseille, France, Tel: 04-91-15-01-89, Fax: 04-91-52-26-07, E-mail: xavier.deparis{at}wanadoo.fr.






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