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Am. J. Trop. Med. Hyg., 79(5), 2008, pp. 670-672
Copyright © 2008 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


Evaluation of Two New Immunochromatographic Assays for Diagnosis of Malaria

Arsène Ratsimbasoa, Laza Fanazava, Rogelin Radrianjafy, Julien Ramilijaona, Hughes Rafanomezantsoa, AND Didier Ménard*
Malaria Research Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar; Primary Health Centre of Ampasimpotsy, Ampasimpotsy, Madagascar

 

ABSTRACT

We assessed the performance of two new commercially available rapid diagnostic tests (RDTs) for malaria (SD Bioline Malaria Ag Pf® test and Ag Pf/Pan® test) in 200 patients with uncomplicated malaria between August and October 2007 in Madagascar. Results of the two RDTs were compared with those obtained by microscopy and real-time polymerase chain reaction. The sensitivity and specificity for detection of Plasmodium falciparum were 93% and 98.9%, respectively, for the SD Bioline Malaria Ag Pf® test and 92.9% and 98.9% for the SD Bioline Malaria Ag Pf/Pan® test. The sensitivity of the SD Bioline Malaria Ag Pf/Pan® test was much lower for detection of other species (63.6%). The sensitivity of the two new assays decreased to 77.3% at parasitemia levels < 100 parasites/µL for detection of P. falciparum.

Received May 21, 2008. Accepted for publication July 17, 2008.

Acknowledgments: We thank the Ministry of Health of Madagascar for allowing us access to malaria patients and the patients for participating in the study.

Financial support: This study was supported by Kozone, representing Standard Diagnostics Inc. in Madagascar. Authors’ addresses: Arsène Ratsimbasoa, Laza Fanazava, Rogelin Radrianjafy, and Didier Ménard, Malaria Unit Research, Institut Pasteur de Madagascar, Antananarivo, Madagascar. Julien Ramilijaona and Hughes Rafanomezantsoa, Primary Health Centre of Ampasimpotsy, Ampasimpotsy, Madagascar.

* Address correspondence to Didier Ménard, Malaria Research Unit, Institut Pasteur de Madagascar, BP 1274, Antananarivo 101, Madagascar. E-mail: dmenard{at}pasteur.mg

Authors’ addresses: Arsène Ratsimbasoa, Laza Fanazava, Rogelin Radrianjafy, and Didier Ménard, Malaria Research Unit, Institut Pasteur de Madagascar, BP 1274-Antananarivo 101, Madagascar. Julien Ramilijaona and Hughes Rafanomezantsoa, Primary Health Centre of Ampasimpotsy, NGO ASA, Ampasimpotsy, Madagascar.






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