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SSCP Analysis of scnDNA for Genetic Profiling of Aedes aegypti

We characterized genetic profiling markers for Aedes aegypti using single-strand conformation polymorphism (SSCP) analysis of single copy nuclear genes (scnDNA). Nucleotide variations at 18 loci were evaluated in 173 wild Ae. aegypti collected from a single population in northwestern Thailand. We identified seven scnDNAs with polymorphisms sufficient to determine a unique genetic profile for each mosquito examined. Six markers were derived from previously mapped cDNA loci. One marker was developed from a non-coding region of a gene. The number of alleles at each scnDNA locus ranged from 3 to 9. The described scnDNAs can be used to quickly fingerprint large numbers of Ae. aegypti to track the behavior of individual mosquitoes in the field.

Received October 18, 2007. Accepted for publication June 20, 2008.

Acknowledgments: The authors thank C. Meneses for technical guidance with SSCP analysis, Y. Lee for help with Arlequin, and K. Glunt for review of the manuscript.

Financial support: This research was supported by NIH Grants RO1 AI022119 to TWS and AI40308 to GCL.

^* Address correspondence to Thomas W. Scott, Department of Entomology, University of California, Davis, 1 Shields Avenue, Davis, CA 95616. E-mail: twscott{at}ucdavis.edu

Authors’ addresses: Jacklyn Wong, Department of Entomology, University of California, Davis, 1 Shields Avenue, Davis, CA 95616, E-mail: jacwong{at}ucdavis.edu. Frédéric Tripet, Centre for Applied Entomology and Parasitology, Keele University, Staffordshire ST5 5BG, UK, E-mail: f.tripet{at}biol.keele.ac.uk. Jason L. Rasgon, The W. Harry Feinstone Department of Molecular Microbiology and Immunology, and The Johns Hopkins Malaria Research Institute, Johns Hopkins University, Suite E5132, North Wolfe Street, Baltimore, MD 21205, E-mail: jrasgon{at}jhsph.edu. Gregory C. Lanzaro, Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, 1 Shields Avenue, Davis, CA 95616, E-mail: gclanzaro{at}ucdavis.edu. Thomas W. Scott, Department of Entomology, University of California, Davis, 1 Shields Avenue, Davis, CA 95616, E-mail: twscott{at}ucdavis.edu.

Reprint requests: Thomas W. Scott, Department of Entomology, University of California, Davis, 1 Shields Avenue, Davis, CA 95616, E-mail: twscott{at}ucdavis.edu.