AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
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Am. J. Trop. Med. Hyg., 79(2), 2008, pp. 256-261
Copyright © 2008 by The American Society of Tropical Medicine and Hygiene

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Isolation and Identification of a Novel Spotted Fever Group Rickettsia, Strain IG-1, from Ixodes granulatus Ticks Collected on Orchid Island (Lanyu), Taiwan

Kun-Hsien Tsai, Hsi-Chieh Wang, Chun-Hsien Chen, Jyh-Hsiung Huang, Hsiu-Ying Lu, Chien-Ling Su, AND Pei-Yun Shu*
Research and Diagnostic Center, Centers for Disease Control, Department of Health, Taipei, Taiwan, Republic of China

A novel species of spotted fever group (SFG) rickettsia, Rickettsia spp. IG-1, was identified and isolated from adult Ixodes granulatus ticks collected from Orchid Island (Lanyu), an islet located in southeastern Taiwan. Serum samples collected from the rodent host Rattus losea and SFG-positive human serum reacted with IG-1 using an immunofluorescence assay (IFA). Pairwise nucleotide sequence analysis of 16S rRNA (rrs), gltA, ompA, ompB, and sca4 shows that IG-1 belongs to SFG rickettsiae and had the highest nucleotide sequence similarities to Rickettsia slovaca and R. sibirica. Phylogenetic analysis of the ompA, ompB genes and sca4 shows that IG-1 is most closely related to R. honei. The results showed the sequence divergence of this novel isolate from those rickettsiae previously characterized using the criteria proposed by Fournier and others. Whether IG-1 is pathogenic for humans remains to be studied.

Received September 21, 2007. Accepted for publication May 8, 2008.

Acknowledgments: The authors thank Professor Didier Raoult and Jean-Yves Patrice, Unité des rickettsies, Marseille, France, for elaborative training and instruction in rickettsia isolation.

Financial support: This research was supported by Grants DOH95-DC-2010 and DOH95-DC-2019 from the Taiwan CDC.

* Address correspondence to Pei-Yun Shu, Research and Diagnostic Center, Centers for Disease Control, Department of Health, Taipei, Taiwan, Republic of China. E-mail: pyshu{at}cdc.gov.tw

Authors’ addresses: Kun-Hsien Tsai, Hsi-Chieh Wang, Chun-Hsien Chen, Jyh-Hsiung Huang, Hsiu-Ying Lu, Chien-Ling Su, and Pei-Yun Shu, Research and Diagnostic Center, Centers for Disease Control, Department of Health, Taipei, Taiwan, Republic of China.






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Copyright © 2008 by the American Society of Tropical Medicine and Hygiene.