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Am. J. Trop. Med. Hyg., 79(2), 2008, pp. 245-247
Copyright © 2008 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


Genetic Analysis of Echinococcus multilocularis Originating from a Patient with Alveolar Echinococcosis Occurring in Minnesota in 1977

Hiroshi Yamasaki*, Minoru Nakao, Kazuhiro Nakaya, Peter M. Schantz, AND Akira Ito
Department of Parasitology and Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa, Japan; Division of Parasitic Diseases, National Center for Zoonotic, Vectorborne and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

 

ABSTRACT

To date, only a single proven case of autochthonous human alveolar echinococcosis has been recorded in Minnesota in 1977. At that time, echinococcal lesions removed from the patient were experimentally inoculated into voles, and the parasite materials obtained from the voles were preserved as histopathologic specimens for 30 years. In this study, retrospective genetic analysis of larval Echinococcus multilocularis originating in the human case was performed using the histopathologic specimens. DNA was extracted from the hematoxylin and eosin–stained specimens, and mitochondrial cytochrome c oxidase subunit 1 gene (cox1) was amplified by polymerase chain reaction. Subsequently, 20 small fragments (100~216 bp) covering almost the entire sequences (97%) of the cox1 were successfully amplified, and the nucleotide sequence analysis showed that the E. multilocularis isolate from Minnesota was almost identical to an isolate from South Dakota rather than isolates from contiguous Alaska.

Received January 11, 2008. Accepted for publication May 11, 2008.

Financial support: This work was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (14256001; 17256002) and Infection Matrix Special Fund from the Ministry of Education, Japan, to AI. The nucleotide sequence data reported in this paper are available in the DDBJ/GenBank/EMBL databases under accession numbers AB353729 and AB374425.

* Address correspondence to Hiroshi Yamasaki, Department of Parasitology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjukuku, Tokyo 162-8640, Japan. E-mail: hyamasak{at}nih.go.jp

Authors’ addresses: Hiroshi Yamasaki, Department of Parasitology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjukuku, Tokyo 162-8640, Japan, Tel: 81-3-5285-1111. ext. 2200, Fax: 81-3-5285-1173, E-mail: hyamasak{at}nih.go.jp. Minoru Nakao, Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2-1-1-1, Asahikawa 078-8510, Japan, Tel: 81-166-68-2423, Fax: 81-166-68-2429, E-mail: nakao{at}asahikawa-med.ac.jp. Kazuhiro Nakaya, Animal Laboratory for Medical Research, Asahikawa Medical College, Midorigaoka Higashi 2-1-1-1, Asahikawa 078-8510, Japan, Tel: 81-166-68-2683, Fax: 81-166-68-2679, E-mail: nky48{at}asahikawa-med.ac.jp. Peter M. Schantz, Division of Parasitic Diseases, National Center for Zoonotic, Vectorborne and Enteric Diseases, Centers for Disease Control and Prevention, 4700 Buford Highway, Atlanta, GA 30341, Tel: 1-770-488-7767, Fax: 1-770-488-7761, E-mail: pms1{at}cdc.gov. Akira Ito, Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2-1-1-1, Asahikawa 078-8510, Japan, Tel: 81-166-68-2420, Fax: 81-166-68-2429, E-mail: akiraito{at}asahikawa-med.ac.jp.






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