AJTMH ASTMH Job Mart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 78(5), 2008, pp. 721-728
Copyright © 2008 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bhattacharya, A.
Right arrow Articles by Bhasin, V. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bhattacharya, A.
Right arrow Articles by Bhasin, V. K.
Related Collections
Right arrow Malaria

In Vitro Activity of Artemisinin in Combination with Clotrimazole or Heat-treated Amphotericin B against Plasmodium falciparum

Amit Bhattacharya, Lokesh C. Mishra, AND Virendra K. Bhasin*
University of Delhi, Department of Zoology, North Campus Delhi University, Delhi 110007, India

Currently available artemisinin-based combination therapies (ACTs) for malaria are inadequate. There remains an enormous unmet need for alternate artemisinin-based combination therapies. One of the fastest methods to identify promising artemisinin-based combination therapies is to look for synergistic or additive antimalarial interaction between artemisinin and an alternate drug against P. falciparum in vitro. Amphotericin B and clotrimazole are known drugs for treatment of human fungal infections. We repurposed clotrimazole or heat-treated amphotericin B in fixed ratio combination with artemisinin for antimalarial properties. Isobologram results show synergistic/additive interaction in both of the cases at therapeutically safe concentrations. Artemisinin, clotrimazole, and their synergistic combinations also decrease hemozoin production in parasitized erythrocytes. New permeation pathways induced in infected cells remain unaffected by drug combinations as indicated by sorbitol lysis. It would be interesting to extend the studies’ in vivo system.

Received October 22, 2007. Accepted for publication December 28, 2007.

Acknowledgments: This work was supported in part by the Department of Biotechnology, Ministry of Science and Technology, Government of India. A.B. and L.C.M. received fellowship from the Council of Scientific and Industrial Research, New Delhi, India.

* Address correspondence to Virendra K. Bhasin, Department of Zoology, University of Delhi, Delhi 110007, India. E-mail: virendrabhasin{at}hotmail.com

Authors’ addresses: Amit Bhattacharya, Lokesh C. Mishra, and Virendra K. Bhasin, Department of Zoology, University of Delhi, Delhi 110007, India, Telephone: 91-11-27667989, Fax: +91-11-27667524, E-mail: virendrabhasin{at}hotmail.com.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2008 by the American Society of Tropical Medicine and Hygiene.