AJTMH ASTMH MEMBERSHIP INFORMATION: astmh@astmh.org
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 78(3), 2008, pp. 472-478
Copyright © 2008 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Tamay-Segovia, P.
Right arrow Articles by Espinoza, B.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tamay-Segovia, P.
Right arrow Articles by Espinoza, B.
Related Collections
Right arrow Vector Biology
Right arrow Triatomid
Right arrow Triatomine bugs
Right arrow Chagas Disease

Two Triatoma dimidiata Clades (Chagas Disease Vector) Associated with Different Habitats in Southern Mexico and Central America

Paulino Tamay-Segovia, Ricardo Alejandre-Aguilar, Fernando Martínez, Guiehdani Villalobos, Francisco J. Zavala-Díaz de la Serna, Patricia de la Torre, Juan Pedro Laclette, Selene Blum-Domínguez, AND Bertha Espinoza*
Centro de Investigaciones en Enfermedades Tropicales, Universidad Autónoma de Campeche, Campeche, México; Departamento de Parasitología, y Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Distrito Federal, México; Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Distrito Federal, México

Triatoma dimidiata is the only reported Chagas disease vector in Campeche, Mexico. The purpose of this study was to determine the genetic variability of vectors from Campeche coastal and rain forest areas and establish a phylogenetic relationship with other T. dimidiata populations by analyzing the internal transcribed spacer-2 (ITS-2) region. The sequence length of samples from Campeche ranged from 469 to 478 basepairs. The ITS-2 variability among the populations enabled us to classify them into two clades with an 18–22 nucleotide difference. The genetic distance (0.042) between them confirms this divergence. Phylogenetic analysis of gene genealogies confirmed these two clades. Furthermore, the population genetic analyses showed two groups with little genetic similarity or migration between them. One group was associated with the tropical forest area and the other group was associated with a mainly coastal distribution. This correlation was also observed when T. dimidiata from other regions of Mexico and Central America were analyzed.

Received March 14, 2007. Accepted for publication December 6, 2007.

Financial support. This study was supported by the Universidad Autónoma de Campeche, Programa Institucional de Formación de Investigadores, Coordinación General de Posgrado e Investigación–Instituto Politécnico Nacional, y Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica from La Dirección General de Asuntos del Personal Académico–Universidad Nacional Autónoma de México grant no. IN 212806-3. Paulino Tamay-Segovia was supported by a scholarship from the Programa para el Mejoramiento del Profesorado of the Universidad Autónoma de Campeche.

* Address correspondence to Bertha Espinoza, Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito Escolar, Ciudad Universitaria, CP 04510, Distrito Federal, México. E-mail: besgu{at}biomedicas.unam.mx

Authors’ addresses: Paulino Tamay-Segovia and Selene Blum-Domínguez, Centro de Investigaciones en Enfermedades Tropicales, Universidad Autónoma de Campeche, Av. Trueba y Regil S/N, CP, Campeche, Campeche, México, Telephone: 981-81-3-01-76, Fax: 981-81-3-01-71, E-mail: pautamay{at}hotmail.com. Ricardo Alejandre-Aguilar and Francisco J. Zavala-Díaz de la Serna, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala S/N, Col. Casco de Sto. Tomas, CP 11340, Distrito Federal, México, Telephone: 525-57-29-63-00 extensions 62400 and 46209, E-mail: rialejandre{at}yahoo.com.mx and javierzavala{at}yahoo.com. Fernando Martínez, Guiehdani Villalobos, Patricia de la Torre, Juan P. Laclette, and Bertha Espinoza, Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito Escolar, Ciudad Universitaria, CP 04510, Distrito Federal, México, Telephone: 525-56-22-89-43, Fax: 525-56-22-33-69, E-mail: fherxyz @yahoo.com, guiehda{at}yahoo.com.mx, and besgu{at}biomedicas.unam.mx.

Reprint requests: Bertha Espinoza, Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Au-tónoma de México, Circuito Escolar, Ciudad Universitaria, CP 04510, Distrito Federal, México, E-mail: besgu{at}biomedicas.unam.mx.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2008 by the American Society of Tropical Medicine and Hygiene.