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Am. J. Trop. Med. Hyg., 78(2), 2008, pp. 294-297
Copyright © 2008 by The American Society of Tropical Medicine and Hygiene

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CASE REPORT


Molecular Identification of Trypanosoma cruzi I Tropism for Central Nervous System in Chagas Reactivation Due to AIDS

Juan M. Burgos, Sandra Begher, Helder M. Valadares Silva, Margarita Bisio, Tomas Duffy, Mariano J. Levin, Andrea M. Macedo, AND Alejandro G. Schijman*
Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Buenos Aires, Argentina; Unidad Clínica Médica e Infectología, Hospital Ignacio Pirovano, Gobierno de la Ciudad de Buenos Aires, Buenos Aires, Argentina; Departamento de Bioquímica e Imunologia, ICB/UFMG, Belo Horizonte, Brazil

 

ABSTRACT

Trypanosoma cruzi lineages, microsatellite allelic polymorphism, and mithocondrial gene haplotypes were directly typified from peripheral blood and cerebrospinal fluid specimens of a Bolivian patient with Chagas disease with accompanying AIDS and central nervous system severe involvement. Of note, the patient’s blood was infected by a mixture of T. cruzi I and T. cruzi IId/e polyclonal populations while the cerebrospinal fluid showed only a monoclonal T. cruzi I population. Our findings do not corroborate the original assumption of innocuity for T. cruzi I in the southern cone of the Americas and highlight lineage I tropism for central nervous system causing lethal Chagas reactivation.

Acknowledgments: The authors thank R. Teijeiro, H. Lopez Alcoba, J. Levalle, and F. Deccarlini of Hospital Pirovano for management and follow-up of the patient and H. Freilij and J. Altcheh of the Laboratory of Chagas of Hospital Ricardo Gutierrez in Buenos Aires city, for serological confirmation of T. cruzi infection.

Financial support: This project received major support from WHO-TDR ID 20285, Bunge & Born Foundation, CONICET (PIP 5469), PICT 33955 from the National Agency of Science and Technology to AGS, and partial support from CNPq/FAPEMIG to AMM. AGS and MJL are members of CONICET Researcher’s Career and AMM of CNPq. JMB, MB, and TD are research fellows of CONICET and HMSV of CAPES.

Disclaimer: All contributing authors have participated in the study and concur with the submission. No conflicts of interests have been disclosed. The data presented in this manuscript have not been and will not be submitted for publication elsewhere.

* Address correspondence to Alejandro G. Schijman, Laboratorio de Biología Molecular de la Enfermedad de Chagas, INGEBI-CONICET, Vuelta de Obligado 2490, Buenos Aires 1428, Argentina. E-mail: schijman{at}dna.uba.ar

This study fulfilled all criteria required by the Medical Code of Ethics and the Helsinki II statement and was approved by Ethical Committee. Written informed consent was obtained from the patient.

Authors’ addresses: Juan M. Burgos, Margarita Bisio, Tomas Duffy, Mariano J. Levin, and Alejandro G. Schijman, Laboratorio de Biología Molecular de la Enfermedad de Chagas, INGEBI-CONICET, Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina, Telephone: 05411-47832871 ext. 50, Fax: 05411-47868578, E-mail: schijman{at}dna.uba.ar. Sandra Begher, Hospital Pirovamo, Monroe 3555, Capital Federal, Argentina. Helder M. Valadares Silva and Andrea M. Macedo, Universidade Federal de Mina Gerais, Av Antonio Carlos 6627, Belo Horizonte, HG, Brazil.






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