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Rapid Active Assay for the Detection of Antibodies to West Nile Virus in Chickens

To reduce the assay time for detecting virus-specific antibodies in serum, we developed microarray-based active immunoassay techniques for detecting West Nile virus (WNV)-specific IgM molecules in chicken blood. The assay uses electrophoretic concentration of IgM molecules onto WNV antigens arrayed on a dialysis membrane followed by detection of bound IgM molecules with functionalized magnetic beads as active labels. This assay takes only 15 minutes and has the same sensitivity as a commercially available human WNV IgM antibody-capture enzyme-linked immunosorbent assay (commonly called a MAC-ELISA) modified for use with chicken sera.

Received August 22, 2007. Accepted for publication October 21, 2007.

Acknowledgments: The authors gratefully acknowledge the valuable suggestions and editorial comments of T. Born and T. Y. Morozova.

Financial support: This study was supported by DOE Grant DE-F C52-04NA25455.

Disclaimer: In conducting research using animals, the investigators adhered to the "Guide for the Care and Use of Laboratory Animals," as prepared by the Committee on Care and Use of Laboratory Animals of the Institute of Laboratory Animal Resources, National Research Council (NIH Publication 86-23, Revised 1996). The facilities are fully accredited by the Association for Assessment and Accreditation of Laboratory Animal Care, International. The views of the authors do not purport to reflect the positions of the Department of the Army or the Department of Defense.

^* Address correspondence to M. J. Turell, Virology Division, USAMRIID, 1425 Porter Street, Fort Detrick, MD 21702-5011. E-mail: mturell{at}erols.com

Authors’ addresses: Stephanie S. Groves, The National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, VA 20110, Telephone: 709-993-9493, E-mail: sgroves1{at}gmu.edu. Michael J. Turell, Virology Division, US Army Medical Research Institute of Infectious Diseases, 1425 Porter St., Fort Detrick, MD 21702-5011, Telephone: 301-619-4921, E-mail: michael.turell{at}amedd.army.mil. Charles L. Bailey, The National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, VA 20110, Telephone: 703-993-4271, E-mail: cbailey2{at}gmu.edu. Victor N. Morozov, The National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, VA 20110, Telephone: 703-993-4294, E-mail: vmorozov{at}gmu.edu, and Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia.

Reprint requests: Victor N. Morozov, The National Center for Bio-defense and Infectious Diseases, George Mason University, Manassas, VA 20110. E-mail: vmorozov{at}gmu.edu.

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