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Characterization of a Dengue Patient Cohort in Recife, Brazil

From 2004 to 2006, 658 patients with suspected dengue virus infections were enrolled in a clinical dengue cohort established in Recife, Pernambuco, located at the northeastern region of Brazil. A total of 2,364 blood samples were collected, and serum, plasma, and cells were cryopreserved. Among the suspected cases, 354 (54%) were confirmed as acute DENV-3 infection based on reverse transcription-polymerase chain reaction, virus isolation, and ELISA-IgM. According to WHO criteria, 29.4% of the positive acute cases were classified as dengue fever (DF) and 8.2% of the cases as dengue hemorrhagic fever (DHF), grade 1 or 2. The DHF cases represent 100% of those confirmed in Recife during the period of the study. The dengue cases that did not fulfill the definition of either DHF or DF were classified as DF complicated and accounted for 44.0% of the cases. All the acute cases were classified as either primary or secondary acute dengue virus infections. Secondary infection was predominant in patients with DF; however, there was no predominance of either primary or secondary infections in patients with DHF.

Received May 18, 2007. Accepted for publication September 5, 2007.

Acknowledgments: We thank all the staff of the Hospital Esperança, Hospital Santa Joana, and Instituto Materno Infantil, Recife, for valuable cooperation with this project.

Financial support: The authors acknowledge the support of the National Institute of Allergy and Infectious Diseases (NIAID/NIH), Grant U19 AI56541, and the additional support of the Brazilian National Research Council (CNPq, Eduardo JM Nascimento’s postdoctoral scholarship 150911/2003-3) and PDTIS RVR09-FIOCRUZ.

^* Address correspondence to Ernesto T. A. Marques, Jr, 725 Wolf Street, Biophysics Build. Room 307A, Baltimore, MD 21205. E-mail: emarques{at}jhmi.edu

Note: Supplementary table 1: " Laboratory results of dengue hemorrhagic fever cases" and Supplementary table 2: " Hemaglutination inhibition antibodies results from the dengue hemorrhagic fever cases" appear online at www.ajtmh.org.

Authors’ addresses: Marli Tenório Cordeiro, Laboratório de Virologia e Terapia Experimental, Centro de Pesquisas Aggeu Magalhães, Fiocruz. Av. Moraes Rego s/n, Campus da UFPE, Cidade Universitária, 50670–420, Recife, PE, Brazil, Telephone: 55 81 2101–2554; Fax: 55 81 21012651; Laboratório Central de Saúde Pública, Secretaria de Saúde do Estado de Pernambuco, Praça Oswaldo Cruz s/n, Boa Vista, 50050–210, Recife, PE, Brazil, E-mail: marli{at}cpqam.fiocruz.br. Ana Maria Silva, Laboratório de Virologia e Terapia Experimental do Centro de Pesquisas Aggeu Magalhães, Fiocruz. Av. Moraes Rego, Campus da UFPE, Cidade Universitária, 50670–420, Recife, PE, Brazil, Telephone: 55 81 2101–2554, Fax: 55 81 21012651, E-mail: anasilva{at}cpqam.fiocruz.br. Carlos A. A. Brito, Laboratório de Virologia e Terapia Experimental, Centro de Pesquisas Aggeu Magalhães, Fiocruz. Av. Moraes Rego, Campus da UFPE, Cidade Universitária, 50670–420, Recife, PE, Brazil, Telephone: 55 81 2101–2554, Fax: 55 81 21012651; Departamento de Medicina Clínica, Universidade Federal de Pernambuco (UFPE), Av. Moraes Rego s/n, Campus da UFPE, Cidade Universitária, 50070–490, Recife, PE, Brazil; Hospital Esperança, Rua Francisco Alves, 887, Ilha do Leite, 50070–490, Recife, PE, Brazil. Telephone: 55 81 2122–4764, E-mail: cbrito{at}cpqam.fiocruz.br. Eduardo J. M. Nascimento, Laboratório de Virologia e Terapia Experimental, Centro de Pesquisas Aggeu Magalhães, Fiocruz. Av. Moraes Rego s/n, Campus da UFPE, Cidade Universitária, 50670–420, Recife, PE, Brazil; Department of Medicine, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, E-mail: enacimento{at}cpqam.fiocruz.br, enascim2{at}jhmi.edu. Maria Cecília F. Magalhães and Georgia F. Guimarães, Laboratório de Virologia e Terapia Experimental, Centro de Pesquisas Aggeu Magalhães, Fiocruz. Av. Moraes Rego s/n, Campus da UFPE, Cidade Universitária, 50670–420, Recife, PE, Brazil. Telephone: 55 81 2101–2554, Fax: 55 81 21012651, E-mails: cecimag{at}cpqam.fiocruz.br and georgia{at}cpqam.fiocruz.br. Norma Lucena-Silva, Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fiocruz. Av. Moraes Rego s/n, Campus da UFPE, Cidade Universitária, 50670–420, Recife, PE, Brazil, Telephone: 55 81 21012640, E-mail: nlucena{at}cpqam.fiocruz.br. Eduardo M. Freese de Carvalho, Departamento Saúde Coletiva do Centro de Pesquisas Aggeu Magalhães, Fiocruz. Av. Moraes Rego, Campus da UFPE, Cidade Universitária, 50670–420, Recife, PE, Brazil, Telephone: 55 81 2101–2593, Fax: 55 81 21012651, E-mail: freese{at}cpqam.fiocruz.br.

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