AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
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Am. J. Trop. Med. Hyg., 77(6), 2007, pp. 1087-1090
Copyright © 2007 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


An Agar Plate Method for Culturing Hookworm Larvae: Analysis of Growth Kinetics and Infectivity Compared With Standard Coproculture Techniques

Daniel Reiss, Lisa M. Harrison, Richard Bungiro, AND Michael Cappello*
Program in International Child Health, Departments of Pediatrics and Epidemiology and Public Health, and Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut

 

ABSTRACT

An agar plate (AP) method has been developed for culturing infectious larvae of the hookworm Ancylostoma ceylanicum. The third-stage larvae reared using the AP method displayed similar morphology to those cultured using Baermann or Harada-Mori coproculture techniques. The yield of viable larvae from the AP method (50%) was comparable to that of the Baermann (47%), and both were superior to Harada-Mori (2.1%). Third-stage larvae cultured by the AP method established patent infection in naturally permissive laboratory hosts, although the yield of adult worms was reduced compared with animals infected with L3 obtained by Baermann culture. The AP method is useful for defining growth requirements for hookworm development, as well as characterizing the effects of bacterially expressed compounds on hookworm larvae in vivo.

Received May 7, 2007. Accepted for publication August 15, 2007.

Acknowledgments: The authors thank Lisa DiFedele, Keke Fairfax, and George Porter for technical assistance and Michael Stern for providing NGM plates.

Financial support: This work was supported by NIH Grants AI58980, AI47929, and AI57855.

* Address correspondence to Michael Cappello, Yale Child Health Research Center, 464 Congress Avenue, New Haven, CT 06520. E-mail: michael.cappello{at}yale.edu

Authors’ addresses: Daniel Reiss, Lisa Harrison, Richard Bungiro, and Michael Cappello, 464 Congress Avenue, New Haven, CT 06520. Tel: 203-737-4320, Fax: 203-737-5972, E-mail: Michael.cappello{at}yale.edu.






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Copyright © 2007 by the American Society of Tropical Medicine and Hygiene.