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Trypanocidal and Leishmanicidal Activities of Flavonoids from Argentine Medicinal Plants

In vitro trypanocidal and leishmanicidal activities of the flavonoids hispidulin, from Ambrosia tenuifolia, and santin, from Eupatorium buniifolium, are reported. A sensitive technique that takes advantage of (³H)thymidine uptake by dividing trypanosomatids has been adjusted for quantification of the parasiticidal effect of the natural products. The IC₅₀ values for hispidulin and santin on Trypanosoma cruzi epimastigotes were 46.7 and 47.4 µM, respectively. On trypomastigotes, the IC₅₀ values were 62.3 µM for hispidulin and 42.1 µM for santin. Hispidulin was more active than santin on promastigotes of Leishmania mexicana (IC₅₀ = 6.0 µM versus 32.5 µM). No cytotoxic activity was observed on lymphoid cells, making hispidulin and santin potential lead compounds for the development of new natural drugs. This is the first report on the trypanocidal and leishmanicidal activities of these flavonoids and on the presence of santin in E. buniifolium.

Received January 8, 2007. Accepted for publication June 17, 2007.

Acknowledgments: The technical assistance of Mr. Jerónimo Ulloa is gratefully acknowledged.

Financial support: This investigation was supported in part by PICT 2002 05-11240 (Agencia Nacional de Promoción Científica y Tecnológica), PIP 02419 (Consejo Nacional de Investigaciones Científicas y Técnicas), UBA-SECYT B101 and PICT 12216 (Agencia Nacional de Promoción Científica y Tecnológica.

^* Address correspondence to Liliana Muschietti, Cátedra de Farmacognosia, Facultad de Farmacia y Bioquímica, Junín 956, 2 Piso, Universidad de Buenos Aires, Buenos Aires 1113, Argentina. E-mail: lmusch{at}ffyb.uba.ar

Authors’ addresses: V. Sülsen, F. Redko, C. Anesini, J. Coussio, V. Martino, and L. Muschietti, Cátedra de Farmacognosia, (IQUIMEFA)–(UBA-CONICET), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. S. Cazorla, F. Frank, and E. Malchiodi, Cátedra de Inmunología, (IDEHU)–(UBA-CONICET), Facultad de Farmacia y Bioquímica; Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina. Junín 956, Buenos Aires 1113, Argentina.

Reprint requests: Liliana Muschietti, Cátedra de Farmacognosia, (IQUIMEFA)–(UBA-CONICET), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 2°P (113), Buenos Aires, Argentina, Telephone: +54 11 4964-8247, Fax: +54 11 4508-3642, E-mail: lmusch{at}ffyb.uba.ar.

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