HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Salas-Benito, J. Articles by del Angel, R. M. Search for Related Content PubMed PubMed Citation Articles by Salas-Benito, J. Articles by del Angel, R. M. Related Collections Cell Biology Pathogenesis Dengue

Evidence that the 45-kD Glycoprotein, Part of a Putative Dengue Virus Receptor Complex in the Mosquito Cell Line C6/36, Is a Heat-Shock–Related Protein

Dengue virus (DENV) is transmitted to humans by mosquitoes of the genus Aedes. Although several molecules have been described as part of DENV receptor complex in mosquito cells, none of them have been identified. Our group characterized two glycoproteins (40 and 45 kD) as part of the DENV receptor complex in C6/36 cells. Because identification of the mosquito cell receptor has been unsuccessful and some cell receptors described for DENV in mammalian cells are heat-shock proteins (HSPs), the role of HSPs in DENV binding and infection in C6/36 cells was evaluated. Our results indicate that gp45 and a 74-kD molecule (p74), which interact with DENV envelope protein, are immunologically related to HSP90. Although p74 is induced by heat shock, gp45 apparently is not. However, these proteins are relocated to the cell surface after heat-shock treatment, causing an increase in virus binding without any effect on virus yield.

Received July 9, 2006. Accepted for publication April 27, 2007.

Acknowledgments: We thank Matilde García Espitia and Fernando Medina for technical assistance, Victor Hugo Reynoso for assistance with flow cytometric analysis, and Ivan Galván for assistance with confocal microscopy.

Financial support: This work was supported by a fellowship from Comisión de Operación y Fomento de Actividades Académicas of the Instituto Politénico Nacional (IPN) to Juan Salas-Benito, and from Consejo Nacional de Ciencia y Tecnología (39946-Q) and Secretaría de Investigación y Posgrado-IPN (CGPI 20050605). Jorge Reyes del Valle was supported by a scholarship from the National Researcher System.

^* Address correspondence to Juan Salas-Benito, Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera #239, Frac. la Escalera Ticomán, Mexico City, Mexico. E-mail: e-mail: jsalasb{at}yahoo.com

Authors’ addresses: Juan Salas-Benito and Mariana Salas-Benito, Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera #239 Frac. La Escalera Ticomán. Mexico City, Mexico CP 07320, Telephone: 52-55-57-29-6000 extension 55536, E-mail: jsalasb{at}yahoo.com. Jorge Reyes-Del Valle, Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN, 55905. Ivonne Ceballos-Olvera, Clemente Mosso, and Rosa M. del Angel, Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. Instituto Politécnico Nacional, #2508 Col. San Pedro Zacatenco, Mexico City, Mexico, CP 07360, Telephone: 52-55-5061-3800 extension 5647.