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RESISTANCE TO DEHYDRATION BETWEEN BOUTS OF BLOOD FEEDING IN THE BED BUG, CIMEX LECTULARIUS, IS ENHANCED BY WATER CONSERVATION, AGGREGATION, AND QUIESCENCE

To determine how the bed bug, Cimex lectularius, survives in a dry environment for many months without feeding, water-balance characteristics were compared for all stages from first-instar nymphs to adults. This species is characterized by a low net transpiration rate averaging < 0.2%/h, high tolerance for dehydration (30–40% loss in body water), and an impermeable cuticle as indicated by a high critical transition temperature (CTT) in the 35–40°C range, implying that this insect is adapted for desiccation-hardiness. The capacity of adults to survive for 2 weeks at 0.00a_v (a_v = % RH/100) with no access to food or water exemplifies this trait. In contrast to more mature stages, first-instar nymphs contain more water, lose water at a faster rate, experience abrupt water loss at a lower temperature, and survive less time in dry air, suggesting that this stage is the most sensitive to water stress. This insect relies on blood to replenish water stores; none of the stages examined have the capacity to absorb water vapor (critical equilibrium activity, CEA 0.99a_v), and they drank only sparingly when offered free water. As the bed bugs progress through their development, they gradually reduce their water requirements while increasing their desiccation resistance. Surviving water stress is considerably enhanced behaviorally by quiescence, characterized by prolonged periods of inactivity, and by the formation of clusters that generate a water-conserving group effect.

Received October 31, 2006. Accepted for publication January 6, 2007.

Acknowledgment: We are grateful to George Keeney, Department of Entomology, The Ohio State University, for maintenance of the bed bug colonies used in these experiments.

Financial support: This work was supported in part by a grant from the National Institutes of Health (ROI AI058279).

^* Address correspondence to Joshua B. Benoit, Department of Entomology, The Ohio State University, 318 W. 12th Ave., Columbus, OH 43210. E-mail: benoit.8{at}osu.edu

Authors’ addresses: Joshua B. Benoit and David L. Denlinger, Department of Entomology, The Ohio State University, 318 W. 12th Ave., Columbus, OH 43210, Telephone: +1 (614) 247-5097, Fax: +1 (614) 292-2180, E-mail: benoit.8{at}osu.edu. Nicholas A. Del Grosso and Jay A. Yoder, Department of Biology, Wittenberg University, Ward St. at Wittenberg Ave., Springfield, OH 45501.

Reprint requests: Joshua B. Benoit, Department of Entomology, The Ohio State University, 318 W. 12th Ave., Columbus, OH 43210, Telephone: +1 (614) 247-5097, Fax: +1 (614) 292-2180, E-mail: benoit.8{at}osu.edu.