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EVALUATION OF FAS2-ELISA FOR THE SEROLOGICAL DETECTION OF FASCIOLA HEPATICA INFECTION IN HUMANS

The performance of Fas2-ELISA for the diagnosis of Fasciola hepatica infection in children living in areas of high endemicity for fascioliasis in the Peruvian Andes is analyzed. Fas2-ELISA is based on the detection of circulating IgG antibodies elicited in infected individuals against a F. hepatica antigen termed Fas2. The study was conducted in three Andean localities, Huertas-Julcan in Junin, Asillo in Puno, and Cajamarca, with a total population of 634 children in an age range 1 to 16 years old. Child fascioliasis prevalence was 21.1% in Huertas-Julcan, 25.4% in Asillo, and 24% in Cajamarca, estimated by coprological inspection. The seroprevalence of F. hepatica infection, determined by Fas2-ELISA, was 27.8% in Huertas-Julcan, 44.6% in Asillo, and 29.1% in Cajamarca. The overall sensitivity of Fas2-ELISA was 92.4%, the specificity 83.6%, and the negative predictive value 97.2%. No association between OD₄₅₀ Fas2-ELISA and infection intensity measured by egg counting was observed. Results show that Fas2-ELISA is a highly sensitive immunodiagnostic test for the detection of F. hepatica infection in children living in human fascioliasis endemic areas.

Received September 3, 2006. Accepted for publication November 21, 2006.

Acknowledgments: The authors thank the people of Huertas-Julcan, Asillo, and Cajamarca, who made it possible to perform this study. We are grateful to Rosendo Ccenta for technical support.

Financial support: E. Chavarry and V. Neyra are graduate students funded by the agreement between the Consortium of Francophones Universities of Belgium (CIUF) and Universidad Peruana Cayetano Heredia (UPCH). S. Saez was funded by a fellowship from the Alfa Network of Parasitology (Red Alfa de Parasitología - RAPAR; Contract No. ALR/B7-3011/94.04-7.0012.9) of the ALFA Programme of the Commission of the European Communities (DG I), Brussels, EU. Research work of the Valencia team funded by the Red de Investigación de Centros de Enfermedades Tropicales – RICET (Project No. C03/04 of the Programme of Redes Temáticas de Investigación Cooperativa) of the Fondo de Investigación Sanitaria (FIS), Spanish Ministry of Health, Madrid, Spain, and by Project No. 3006/1999 of the Dirección General de Cooperación para el Desarrollo, Presidencia de Gobierno de la Generalitat Valenciana, Valencia, Spain.

^* Address correspondence to Jose R. Espinoza, Unit of Molecular Biotechnology, Facultad de Ciencias y Filosofia, Universidad Peruana Cayetano Heredia, PO Box 4314, Lima 100, Peru. E-mail: jrespinoza{at}upch.edu.pe

Authors’ addresses: Jose R. Espinoza, Vicente Maco, Luis Marcos, Victor Neyra, Elizabeth Chavarry and Maria Cecilia Huaman, Molecular Biotechnology Unit, Facultad de Ciencias y Filosofia, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, S.M.P., Lima 31, Peru, E-mail: jrespinoza{at}upch.edu.pe. Angelica Terashima, Frine Samalvides, and Eduardo Gotuzzo, Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia. Sandra Saez, M. Dolores Bargues, M. Adela Valero, and Santiago Mas-Coma, Departamento de Parasitologia, Facultad de Farmacia, Universidad de Valencia, Av. Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain.

Reprint requests: Jose R. Espinoza, Unit of Molecular Biotechnology, Facultad de Ciencias y Filosofia, Universidad Peruana Cayetano Heredia, PO Box 4314, Lima 100, Peru, Telephone: + 511 319 0000 ext. 2502, Fax: + 511 319 0024, E-mail: jrespinoza{at}upch.edu.pe.

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