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Am. J. Trop. Med. Hyg., 76(5), 2007, pp. 855-859
Copyright © 2007 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


RARE PLASMODIUM FALCIPARUM MEROZOITE SURFACE PROTEIN 1 19-KDA (MSP-119) HAPLOTYPES IDENTIFIED IN MALI USING HIGH-THROUGHPUT GENOTYPING METHODS

SHANNON L. TAKALA, DAVID L. SMITH, MAHAMADOU A. THERA, DRISSA COULIBALY, OGOBARA K. DOUMBO, AND CHRISTOPHER V. PLOWE*
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Fogarty International Center, National Institutes of Health, Bethesda, Maryland; Malaria Research and Training Center, University of Bamako, Bamako, Mali

 

ABSTRACT

Genetic diversity in malaria vaccine antigens may compromise malaria vaccine efficacy, so it is important to understand this diversity and the processes that generate it. By applying new high-throughput genotyping methods to a large sample of infections from Mali (N = 1369), seven new 19-kDa merozoite surface protein 1 (MSP-119) haplotypes were identified. Herein we report the sequences of these new haplotypes and discuss their possible origins. Although they are present in < 1% of the samples examined, the existence of these rare haplotypes reveals a greater degree of diversity at this locus than previously reported and highlights the potential for Plasmodium to evolve under selective pressure from the immune system and from such interventions as vaccines and drugs.

Received October 9, 2006. Accepted for publication January 8, 2007.

Acknowledgments: The authors thank the population of Bandiagara, Mali, for their continued participation in our studies, as well as the regional and district health authorities of Bandiagara, Mali for their continued support. We also thank Dr. Alan Shuldiner and the Division of Endocrinology, Diabetes, and Nutrition, University of Maryland School of Medicine, for use of the Pyrosequencer. In addition, we acknowledge Dr. Ananias Escalante for helpful comments on the manuscript.

Financial support: This study was funded by NIAID (N01AI85346) and the USAID Malaria Vaccine Program.

* Address correspondence to Christopher V. Plowe, Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201. E-mail: cplowe{at}medicine.umaryland.edu

Authors’ addresses: Shannon L. Takala and Christopher V. Plowe, Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD, Telephone: +1 (410) 706-3082, Fax: +1 (410) 706-6205, E-mail: cplowe{at}medicine.umaryland.edu. David L. Smith, Fogarty International Center, National Institutes of Health, Bethesda, MD. Mahamadou A. Thera, Drissa Coulibaly, and Ogobara K. Doumbo, Malaria Research and Training Center, University of Bamako, Bamako, Mali.






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