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Am. J. Trop. Med. Hyg., 75(6), 2006, pp. 1103-1105
Copyright © 2006 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


EVALUATION OF A BOOSTED-P24 ANTIGEN ASSAY FOR THE EARLY DIAGNOSIS OF PEDIATRIC HIV-1 INFECTION IN CAMBODIA

JANIN NOUHIN AND MARIE NGUYEN*
Institut Pasteur du Cambodge, Phnom Penh, Cambodia

 

ABSTRACT

Methods for early diagnosis of pediatric HIV-1 infection (DNA-polymerase chain reaction [PCR], RNA quantification, viral cultures) are expensive. Most Cambodian infants wait 18 months for HIV serologic tests. We observed that boosted-p24-antigen profile assay, with performances similar to viral cultures and costs similar to DNA-PCR, is easier to perform and could readily be set up in resource-poor settings.

Received April 19, 2006. Accepted for publication August 19, 2006.

Acknowledgments: We are grateful to R. Sutthent and C. Watcharapin for technical help and to J.-M. Reynès and Y. Henin for critical reading of the manuscript. The authors thank Perkin-Elmer and their representatives I. Cabruja and S. Guibert for the gift of the HIV-1 p24 core profile ELISA and ELAST ELISA amplification kits and M. Giacomini and S. Bertone for the gift of the Quantikin Detection System software and RS 232 cable. We acknowledge L. Chartier for help in statistics.

* Address correspondence to Marie Nguyen, Institut Pasteur, PFID, 25-28, rue du Docteur Roux, 75724 Paris Cedex 15, France. E-mail: marie{at}pasteur.fr

Authors’ addresses: Janin Nouhin, Institut Pasteur du Cambodge, 5 Boulevard Monivong, BP 983, Phnom Penh, Cambodia. Marie Nguyen, Institut Pasteur, PFID, 25-28, rue du Docteur Roux, 75724 Paris Cedex 15, France, Telephone: 33-1-45-68-84-97, Fax: 33-1-45-68-89-49, E-mail: marie{at}pasteur.fr.




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J. C. Patton, A. H. Coovadia, T. M. Meyers, and G. G. Sherman
Evaluation of the Ultrasensitive Human Immunodeficiency Virus Type 1 (HIV-1) p24 Antigen Assay Performed on Dried Blood Spots for Diagnosis of HIV-1 Infection in Infants
Clin. Vaccine Immunol., February 1, 2008; 15(2): 388 - 391.
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Copyright © 2006 by the American Society of Tropical Medicine and Hygiene.