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Am. J. Trop. Med. Hyg., 75(5), 2006, pp. 871-879
Copyright © 2006 by The American Society of Tropical Medicine and Hygiene

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Right arrow Chagas Disease

CONGENITAL CHAGAS DISEASE IN BOLIVIA IS NOT ASSOCIATED WITH DNA POLYMORPHISM OF TRYPANOSOMA CRUZI

MYRNA VIRREIRA, CRISTINA ALONSO-VEGA, MARCO SOLANO, JUAN JIJENA, LAURENT BRUTUS, ZULEMA BUSTAMANTE, CARINE TRUYENS, DOMINIQUE SCHNEIDER, FAUSTINO TORRICO, YVES CARLIER, AND MICHAL SVOBODA*
Laboratoire de Chimie Biologique, Faculté de Médecine, Université Libre de Bruxelles (U.L.B.), Brussels, Belgium; Facultad de Medecina, Universidad Mayor de San Simon (U.M.S.S.), Cochabamba, Bolivia; Unidad de Neonatologia, Hospital "San Juan de Dios", Tarija, Bolivia; Institut de Recherche pour le Développement (IRD, France), Mother and Child Health Research Unit (UR10), La Paz, Bolivia; Laboratoire de Parasitologie, Faculté de Médecine, Université Libre de Bruxelles (U.L.B.), Brussels, Belgium

This study aims to typify the Trypanosoma cruzi (sub)lineage(s) in umbilical cord blood of congenitally infected Bolivian newborns, using PCR amplifications of "Region Markers", mini-exon or kDNA fragments followed by hybridization or sequencing. New probes were also designed to distinguish three variants within the TcIId sublineage. The IIb, IId, or IIe T. cruzi sublineages, as well as different variants of the IId sublineage, were detected in infected neonates, whereas mixed infections were not found. The frequencies of the IId sublineage were similar in neonates (95.1%) and adults of the same area (94.1%). The IId-infected newborns displayed either asymptomatic, or severe and fatal clinical forms of congenital Chagas disease, as well as low or high parasitemia. Altogether these data show that T. cruzi DNA polymorphism, based on the presently available markers, is not associated with the occurrence of congenital infection or the development of severe clinical forms of congenital Chagas disease.

Received March 30, 2006. Accepted for publication May 26, 2006.

Acknowledgments: The authors thank Marisol Cordova and the staff of the hospital materno-infantil German Urquidi (Cochabamba, Bolivia), Bertha Mendoza, Claudia Bernal, and the staff of the maternity Odon Ortega Flores of Yacuiba (Bolivia) and of the "Unidad de Neonatologia" del Hospital Universitario San Juan de Dios (Tarija, Bolivia) for the management of patients; Mary Cruz Torrico, Patricia Rodriguez, Miguel Guzman, Rudy Parrado, Amilcar Flores, and Tatiana Tellez (CUMETROP/LABIMED, U.M.S.S., Cochabamba, Bolivia), Jorge Postigo (UR010-IRD, La Paz, Bolivia) and Dolores Rangel y Nelly Aguado (Hospital Universitario San Juan de Dios and Laboratorio de Epidemiologia-SEDES, Tarija, Bolivia) for the serological and parasitological diagnosis of patients. C. Alonso-Vega is fellow of the "Association pour la promotion de l’éducation et la formation à l’étranger" (APEFE, "Communauté Française de Belgique"). This study was supported by the Conseil Interuniversitaire de la Communauté Française de Belgique (CIUF), the « Fonds National de la Recherche Scientifique Médicale » (Belgium, conventions 3.4.595.99/3.4.615.05), and the "Institut de Recherche pour le Développement" (IRD, France). Part of the present data have been presented to the international colloquium: "Infeccion congenita por Trypanosoma cruzi: desde los mecanismos de transmision hasta una estrategia de diagnostico y control", held on November 6–8, 2002, at Cochabamba, Bolivia.

* Address correspondence to Michal Svoboda, Laboratoire de Chimie Biologique, Faculté de Médecine, U.L.B. 808 route de Lennik CP 611, B-1070 Bruxelles, Belgium. E-mail: msvobod{at}ulb.ac.be

Authors’ addresses: Myrna Virreira and Zulema Bustamante, Faculté de Médecine, Université Libre de Bruxelles, 808 route de Lennik CP 611, B-1070 Brussels, Belgium, E-mails: mvirreir{at}ulb.ac.be and ciuf2{at}bio.umss.edu.bo. Cristina Alonso-Vega, Marco Solano, and Faustino Torrico, Facultad de Medecina, Universidad Mayor de San Simon (U.M.S.S.), Cochabamba, Bolivia, E-mails: cristina{at}med.umss.edu.bo, msolano{at}med.umss.edu.bo, and ftorrico{at}med.umss.edu.bo. Juan Jijena, Unidad de Neonatologia, Hospital "San Juan de Dios", Tarija, Bolivia, E-mail: jujijena{at}cosett.com.bo. Laurent Brutus and Dominique Schneider, Mother and Child Health Research Unit (UR10), La Paz, Bolivia, E-mails: laurentbrutus{at}club-internet.fr and dschneider{at}club-internet.fr. Carine Truyens and Yves Carlier, Laboratoire de Parasitologie, Faculté de Médecine, Université Libre de Bruxelles (U.L.B.), Brussels, Belgium, E-mails: ctruyens{at}ulb.ac.be and ycarlier{at}ulb.ac.be. Michal Svoboda Laboratoire de Chimie Biologique, Faculté de Médecine, U.L.B. 808 route de Lennik CP 611, B-1070 Brussels, Belgium, Telephone: 32-2-555-62-25, Fax: 32-2-555-62-30, E-mail: msvobod{at}ulb.ac.be.




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M. Virreira, C. Truyens, C. Alonso-Vega, L. Brutus, J. Jijena, F. Torrico, Y. Carlier, and M. Svoboda
Comparison of Trypanosoma cruzi Lineages and Levels of Parasitic DNA in Infected Mothers and Their Newborns
Am J Trop Med Hyg, July 1, 2007; 77(1): 102 - 106.
[Abstract] [Full Text] [PDF]


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Copyright © 2006 by the American Society of Tropical Medicine and Hygiene.