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Am. J. Trop. Med. Hyg., 75(5), 2006, pp. 843-850
Copyright © 2006 by The American Society of Tropical Medicine and Hygiene

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IDENTIFICATION OF A NOVEL GENE ENCODING A SECRETED ANTIGEN 1 OF BABESIA GIBSONI AND EVALUATION OF ITS USE IN SERODIAGNOSIS

HONGLIN JIA, JINLIN ZHOU, HIROMI IKADAI, AYA MATSUU, HIROSHI SUZUKI, IKUO IGARASHI, KOZO FUJISAKI, AND XUENAN XUAN*
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan; School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan

Serum from a dog immunized with blood plasma from a B. gibsoni-infected dog, putatively containing secreted antigens, was used to screen a cDNA expression library. A novel gene encoding BgSA1 was identified from the isolated clones. The serum raised in mice immunized with the recombinant BgSA1 expressed in Escherichia coli could recognize a native parasite protein with a molecular mass of 59 kDa. Comparing with the previously established ELISA with recombinant P50 as antigen, the ELISA with recombinant BgSA1 as the antigen was more sensitive when they were used to detect field samples. Moreover, a sandwich ELISA with anti-BgSA1 antibodies could detect the circulating BgSA1 in a serial blood plasma from a dog experimentally infected with B. gibsoni. These results indicated that BgSA1 could be a useful target for the development of a diagnostic test for the detection of specific antibodies and circulating antigens.

Received March 26, 2006. Accepted for publication May 8, 2006.

Acknowledgments: This work was supported by a grant from The 21st Century COE Program (A-1) and a Grant-in-Aid for Scientific Research, both from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

* Address correspondence to Xuenan Xuan, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. E-mail: gen{at}obihiro.ac.jp

Authors’ addresses: Honglin Jia, Jinlin Zhou, Hiroshi Suzuki, Ikuo Igarashi, Kozo Fujisaki, and Xuenan Xuan, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan, Telephone: 81-155-49-5648, Fax: 81-155-49-5643, E-mails: jhl0627{at}obiniro.ac.jp, jinlin{at}obihiro.ac.jp, and gen{at}obihiro.ac.jp. Hiromi Ikadai and Aya Matsuu, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan.






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Copyright © 2006 by the American Society of Tropical Medicine and Hygiene.