AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
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Am. J. Trop. Med. Hyg., 75(4), 2006, pp. 664-668
Copyright © 2006 by The American Society of Tropical Medicine and Hygiene

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NATURAL ANAPLASMA PHAGOCYTOPHILUM INFECTION OF TICKS AND RODENTS FROM A FOREST AREA OF JILIN PROVINCE, CHINA

WU-CHUN CAO*, LIN ZHAN, JING HE, JANET E. FOLEY, SAKE J. DE VLAS, XIAO-MING WU, HONG YANG, JAN. H. RICHARDUS, AND J. DIK F. HABBEMA
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, People’s Republic of China; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California; Department of Public Health, Erasmus MC, University Medical Center Rotterdam, The Netherlands

Polymerase chain reaction integrated with sequence analysis was carried out to investigate the natural Anaplasma phagocytophilum infection in ticks and rodents from a forest area of Jilin Province, China. Four (4.0%) of 100 Ixodes persulcatus and 2 (0.7%) of 286 Dermacentor silvarum ticks collected by flagging vegetation were positive. Nine (8.8%) of 102 rodents were infected, as well as 2 (2.8%) of 71 I. persulcatus parasitizing on 25 rodents. The nucleotide sequences of 1442-bp A. phagocytophilum 16S rRNA gene amplified from rodents and ticks were identical to each other and to that previously reported in Heilongjiang Province of China (GenBank accession no. AF205140), but different from those of other countries. The sequences of 357-bp partial citrate synthase gene from the above specimens were homologic, and varied from known A. phagocytophilum agents. These findings add new information on the ecologic features of A. phagocytophilum and indicate the threat of anaplasmosis in the area.

Received February 1, 2006. Accepted for publication May 16, 2006.

Acknowledgments: We are grateful to Dr. Rong-man Xu for identification of ticks and reading the manuscript and to Ms. Hai-nan Huang for assistance in tick collection.

Financial support: This study was carried out with financial support from the National Key Technology R & D Program of China (2003BA712a05-01), National Natural Science Foundation of China (No. 39970655), and the Commission of the European Community, as part of the project "Effective and Acceptable Strategies for the Control of SARS and New Emerging Infections in China and Europe" (Contract No. 003824).

* Address correspondence to Wu-chun Cao, Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, People’s Republic of China. E-mail: caowc{at}nic.bmi.ac.cn

Authors’ addresses: Wu-Chun Cao, Lin Zhan, Jing He, Xiao-Ming Wu, and Hong Yang, Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, 20 Dong-Da Street, Fengtai District, Beijing 100071, P. R. China. Janet E. Foley, Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616. Sake J. de Vlas, Jan H. Richardus, and J. Dik F. Habbema, Department of Public Health, Faculty of Medicine and Health Sciences, Erasmus MC, University Medical Center Rotterdam, P.O. Box 2040, 3000 CA Rotterdam, The Netherlands.

Reprint requests: Dr. Wu-Chun Cao, Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, 20 Dong-Da Street, Fengtai District, Beijing 100071, P. R. China, Telephone: (+86) 10-63896082, Fax: (+86) 10-63896082, E-mail: caowc{at}nic.bmi.ac.cn or caowc2000{at}yahoo.com.cn.




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Copyright © 2006 by the American Society of Tropical Medicine and Hygiene.