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Am. J. Trop. Med. Hyg., 75(3), 2006, pp. 563-564
Copyright © 2006 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


A MODIFIED FILTER PAPER CULTURE TECHNIQUE FOR SCREENING OF STRONGYLOIDES STERCORALIS IVERMECTIN SENSITIVITY IN CLINICAL SPECIMENS

PEWPAN M. INTAPAN*, THIDARAT K. PRASONGDEE, PORNTIP LAUMMAUNWAI, KITTISAK SAWANYAWISUTH, SAREE SINGTHONG, AND WANCHAI MALEEWONG
Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Office of Disease Prevention and Control, Region VI, Department of Disease Control, Ministry of Public Health, Khon Kaen, Thailand

 

ABSTRACT

An in vitro test for the detection of ivermectin sensitivity of Strongyloides stercoralis in human clinical specimens was developed. The test has a simple procedure that combines parasite culture with a drug assay. It is based on the principle of the drug effect on larval development test, combined with a modified coproculture using the filter paper culture technique. This does not require parasite isolation, and drug sensitivity can be shown in relationship to the dose. This test can be applied in field surveys for anthelmintic sensitivity and for appropriate drug rotation strategies when drug resistance occurs.

Received February 16, 2006. Accepted for publication May 16, 2006.

Acknowledgments: The authors thank Dr. Mark Roselieb for improving the English language in the manuscript.

Financial support: This research was supported by a grant from Khon Kaen University.

* Address correspondence to Pewpan M. Intapan, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. E-mail: pewpan{at}kku.ac.th

Authors’ addresses: Pewpan M. Intapan, Thidarat K. Prasongdee, Porntip Laummaunwai, and Wanchai Maleewong, Department of Parasitology, Faculty of Medicine and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand. Kittisak Sawanyawisuth, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. Saree Singthong, Office of Disease Prevention and Control, Region VI, Department of Disease Control, Ministry of Public Health, Khon Kaen 40002, Thailand.

Reprint requests: Pewpan M. Intapan, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. E-mail: pewpan{at}kku.ac.th.






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