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Am. J. Trop. Med. Hyg., 75(3), 2006, pp. 486-490
Copyright © 2006 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


ISOLATION OF TWO VACCINIA VIRUS STRAINS FROM A SINGLE BOVINE VACCINIA OUTBREAK IN RURAL AREA FROM BRAZIL: IMPLICATIONS ON THE EMERGENCE OF ZOONOTIC ORTHOPOXVIRUSES

GILIANE S. TRINDADE, ZÉLIA I. P. LOBATO, BETÂNIA P. DRUMOND, JULIANA A. LEITE, RICARDO C. TRIGUEIRO, MARIA I. M. C. GUEDES, FLÁVIO G. DA FONSECA, JOÃO R. DOS SANTOS, CLÁUDIO A. BONJARDIM, PAULO C. P. FERREIRA, AND ERNA G. KROON*
Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Laboratório de Imunologia Celular e Molecular, Centro de Pesquisas René Rachou–FIOCRUZ, Belo Horizonte, Brazil

 

ABSTRACT

Outbreaks of bovine vaccinia disease caused by circulation of Vaccinia virus (VACV) strains have been a common occurrence in Brazil in the recent years, being an important emergent zoonosis. During a single outbreak that took place in 2001, two genetically different VACV strains were isolated and named Guarani P1 virus (GP1V) and Guarani P2 virus (GP2V). Molecular diagnosis was done through restriction fragment length polymorphism (RFLP) of ati gene (A26L) and by sequence analysis of a group of five VACV genes including the C11R, J2R, A56R, B18R, and E3L genes. These findings confirmed the co-circulation of two different Vaccinia virus strains during the same outbreak, raising important questions about the origin, emergence, and circulation of VACV strains in Brazil.

Received February 15, 2006. Accepted for publication May 10, 2006.

Acknowledgments: The authors thank Dr. Marieta Cristina Madureira from Instituto Mineiro de Agropecuária for support during the epidemiologic investigation. We also thank Dr. José Mário Vilela and Dr. Margareth Spangler Andrade from Nanoscopy Laboratory, Fundação Centro Tecnológico de Minas Gerais, for the AFM analysis. The aid of Dr. Fabrício dos Santos, Rodrigo Redondo, and Laboratório de Biodiversidade e Evolução Molecular where all sequences were made is gratefully acknowledged.

Financial support: The CNPq, CAPES, and FAPEMIG provided financial support. E. G. Kroon, C. A. Bonjardim, P. C. P. Ferreira, F. G. Da Fonseca, and J. R. Santos received fellowship from CNPq. M. I. M. C. Guedes received fellowship from FAPEMIG.

* Address correspondence to Erna Geessien Kroon, Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, caixa postal 486, CEP 31270-901 Belo Horizonte, MG, Brazil. E-mail: kroone{at}icb.ufmg.br

Authors’ addresses: Giliane de Souza Trindade, Betânia Paiva Drumond, Juliana Almeida Leite, Ricardo Campos Trigueiro, Maria Isabel Maldonado Coelho Guedes, João Rodrigues dos Santos, Cláudio Antônio Bonjardim, Paulo César Peregrino Ferreira, and Erna Geessien Kroon, Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, caixa postal 486, CEP 31270-901, Belo Horizonte, MG, Brasil, Fax: 55 31 3443-6482, E-mails: gitrindade{at}yahoo.com.br, betaniadrumond{at}uol.com.br, ju{at}icb.ufmg.br, rictrigueiro{at}yahoo.com.br, isabelguedes{at}icb.ufmg.br, santosjr{at}icb.ufmg.br, claubonj{at}icb.ufmg.br, paulocpf{at}icb.ufmg.br, and kroone{at}icb.ufmg.br. Zélia Inês Portela Lobato, Departamento de Medicina Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, CEP 31270-901, Belo Horizonte, MG, Brasil, Fax: 55 31 3443-6482, E-mail: ziplobat{at}vet.ufmg.br. Flávio Guimarães da Fonseca, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, caixa postal 486, CEP 31270-901, Belo Horizonte, MG, Brasil, Fax: 55 31 3443-6482, E-mail: fdafonseca{at}cpqrr.fiocruz.br.




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