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KE
ÍKOVÁ SCHOLZ*
eské Bud
jovice; Department of Microbiology, Universidad Peruana Cayetano Heredia and Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru
Using ITS2 gene sequences, the validity of the tapeworm Diphyllobothrium pacificum (Nybelin, 1931), infecting humans on the Pacific coast of South America and in Japan, was assessed. ITS2 sequences of this cestode differed markedly (sequence similarity 79.0–80.2%) from those of the most common human-infecting cestode, the broad fish tapeworm Diphyllobothrium latum (L.), as well as other four species of Diphyllobothrium, including potential human parasites (D. cordatum, D. dendriticum, and D. lanceolatum) and two species of Spirometra (sequence similarity 77.5–81.9%). Interspecific sequence similarity between all but one (D. pacificum) species was 86.1–99.6%, whereas individual isolates of D. dendriticum and D. ditremum exhibited intraspecific sequence similarity of 97.0–98.0% and 98.2–99.9%, respectively. Phylogenetic trees constructed from ITS2 sequences show a markedly distant position of D. pacificum from other species analyzed and also indicate the possible paraphyly of Spirometra.
Received February 14, 2006. Accepted for publication April 5, 2006.
Acknowledgments: The authors thank the following persons, who kindly provided them specimens for this study: Oleg Ditrich, Lubo
Financial support: This study was financially supported by the Grant Agency of the Czech Republic (Projects 524/04/0342 and 524/03/ H133) and research projects of the Institute of Parasitology, AS CR (Z60220518 and LC522) and the research project of the Faculty of Biological Sciences USB (NSM 6007665801). Stays of two of the authors (R.K. and T.S.) at the Institute of Aquaculture, University of Stirling, Scotland, were supported by the Access to Research Infrastructure Action of the Improving Human Potential Programme of the European Community (contract HPRI-CT-2001-00180), and their expeditions to Peru were realized on the basis of the joint project between the Institute of Parasitology, AS CR, and the Regional Government of Loreto (GOREL), Iquitos.
Pialek, Larisa G. Poddubnaya, Ekaterina N. Protasova, Helmut Sattmann, Andy P. Shinn, and members of the Cysticercosis Working Group, Lima, Peru. Support of the Embassy of Peru in Prague and that of the Czech Republic in Lima, Peru, is also greatly appreciated. The authors also thank Václav Hypa for valuable advice concerning phylogenetic analyses.
* Address correspondence to Tomá Scholz, Institute of Parasitology, Academy of Sciences of the Czech Republic and Faculty of Biological Sciences, University of South Bohemia, Braniovská 31, 370 05 eské Budjovice, Czech Republic. E-mail: tscholz{at}paru.cas.cz
Authors’ addresses: Andrea
ke
íková, Jan Brabec, Roman Kuchta, and Tomá Scholz, Institute of Parasitology, Academy of Sciences of the Czech Republic and Faculty of Biological Sciences, University of South Bohemia, Braniovská 31, 370 05 eské Budjovice, Czech Republic, E-mail: tscholz{at}paru.cas.cz. Juan A. Jiménez and Hector Hugo García, Department of Microbiology, Universidad Peruana Cayetano Heredia and Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru, E-mail: hgarcia{at}jhsph.edu.
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