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Am. J. Trop. Med. Hyg., 74(6), 2006, pp. 941-943
Copyright © 2006 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


RECOVERY AND USE OF PLASMODIUM DNA FROM MALARIA RAPID DIAGNOSTIC TESTS

VINCENT VERON* AND BERNARD CARME*
Medical Parasitology and Mycology Laboratory, Research Team EA 3593, Referral Hospital and School of Medicine, University of West Indies and French Guiana, Cayenne, French Guiana

 

ABSTRACT

The purpose of this study was to show that individual malaria rapid diagnosis tests (MRDTs) could also be used to isolate Plasmodium DNA for genetic studies. We extracted and amplified Plasmodium DNA using two commercial MRDT kits. Phenol/chloroform extraction followed by a nested polymerse chain reaction (PCR) can be used to identify Plasmodium falciparum and Plasmodium vivax from MRDTs. The PCR on MRDT-isolated DNA was more sensitive than antigen capture by MRDT. Satisfactory results were also obtained if older MRDT tests were used, even after long periods of storage at ambient temperature, with no special preservation.

Received June 13, 2005. Accepted for publication November 8, 2005.

Acknowledgments: We thank Brice Rotureau and Mathieu Nacher for assistance in revising the manuscript.

Financial support: This study was supported by the Ministère Français de la Recherche Scientifique Research Team EA 3593.

* Address correspondence to Vincent Veron or Bernard Carme, Laboratoire de Parasitologie et Mycologie Médicale, EA 3593, Centre Hospitalier de Cayenne, Rue des Flamboyants, BP 6006, F-97 306 Cayenne, French Guiana. E-mails: veronivi{at}voila.fr or b.carme{at}wanadoo.fr

Authors’ address: Vincent Veron and Bernard Carme, Laboratoire de Parasitologie et Mycologie Médicale, EA 3593, Centre Hospitalier de Cayenne, Rue des Flamboyants, BP 6006, F-97 306 Cayenne, French Guiana, E-mails: veronvi{at}voila.fr and b.carme{at}wanadoo.fr.






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Copyright © 2006 by the American Society of Tropical Medicine and Hygiene.