HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by TRIPET, F. Articles by LANZARO, G. Search for Related Content PubMed PubMed Citation Articles by TRIPET, F. Articles by LANZARO, G.

A NEW HIGH-PERFORMANCE PCR DIAGNOSTIC FOR THE DETECTION OF PYRETHROID KNOCKDOWN RESISTANCE KDR IN ANOPHELES GAMBIAE

Monitoring the spread of the knockdown resistance allele kdr in areas of extensive pyrethroid use is critical to vector-control projects. Currently available methods for detecting kdr from DNA samples are characterized by poor amplification, time-consuming steps, and primers that exhibit frequent null alleles. We describe a new PCR diagnostic that uses fluorescent primers based on conserved priming sites and enables simple detection of the kdr allele on a sequencer. Using samples from a West African Anopheles gambiae population, we show that the new PCR yielded significantly higher rates of amplification and more accurate estimates of kdr frequency. The method works equally well for the leucine to phenylalanine substitution found in West Africa and the East African leucine to serine substitution.

Received October 18, 2005. Accepted for publication November 8, 2005.

Acknowledgments: The authors thank Claudio Meneses for help with the maintenance of mosquito strains.

Financial support: This project was supported by NIAID/NIH grant AI40308 to G.C.L.

^* Address correspondence to Frederic Tripet, Center for Applied Entomology and Parasitology, School of Life Sciences, Keele University, Staffordshire, ST5 5BG, UK. E-mail: frtripet{at}yahoo.com

Author’s addresses: Frederic Tripet, Jennifer Wright, and Greg Lanzaro, Department of Entomology, University of California Davis, 1 Shields Ave., Davis, CA 95616-8584, E-mails: ftripet{at}ucdavis.edu, jwright{at}ucdavis.edu and glanzaro{at}ucdavis.edu. Frederic Tripet (new address), Center for Applied Entomology and Parasitology, School of Life Sciences, Keele University, Staffordshire, ST5 5BG, UK.

Reprint requests: Frederic Tripet, Center for Applied Entomology and Parasitology, School of Life Sciences, Keele University, Stafford-shire, ST5 5BG, UK, E-mail: frtripet{at}yahoo.com.

This article has been cited by other articles:

				A. Kazanidou, D. Nikou, M. Grigoriou, J. Vontas, and G. Skavdis A Multiplex PCR Assay for Simultaneous Genotyping of kdr and ace-1 Loci in Anopheles gambiae Am J Trop Med Hyg, February 1, 2009; 80(2): 236 - 238. [Abstract] [Full Text] [PDF]

				Y. E. Himeidan, H. Chen, F. Chandre, M. J. Donnelly, and G. Yan Permethrin and DDT Resistance in the Malaria Vector Anopheles arabiensis from Eastern Sudan Am J Trop Med Hyg, December 1, 2007; 77(6): 1066 - 1068. [Abstract] [Full Text] [PDF]

				F. TRIPET, J. WRIGHT, A. CORNEL, A. FOFANA, R. MCABEE, C. MENESES, L. REIMER, M. SLOTMAN, T. THIEMANN, G. DOLO, et al. LONGITUDINAL SURVEY OF KNOCKDOWN RESISTANCE TO PYRETHROID (KDR) IN MALI, WEST AFRICA, AND EVIDENCE OF ITS EMERGENCE IN THE BAMAKO FORM OF ANOPHELES GAMBIAE S.S. Am J Trop Med Hyg, January 1, 2007; 76(1): 81 - 87. [Abstract] [Full Text] [PDF]