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Am. J. Trop. Med. Hyg., 74(4), 2006, pp. 593-597
Copyright © 2006 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


CLONING OF THE BABESIA GIBSONI CYTOCHROME B GENE AND ISOLATION OF THREE SINGLE NUCLEOTIDE POLYMORPHISMS FROM PARASITES PRESENT AFTER ATOVAQUONE TREATMENT

AYA MATSUU*, KAYOKO MIYAMOTO, HIROMI IKADAI, SHOZO OKANO, AND SEIICHI HIGUCHI
Department of Small Animal Internal Medicine 1, Department of Veterinary Parasitology, and Department of Small Animal Surgery 3, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan

 

ABSTRACT

We determined the nucleotide sequence of the Babesia gibsoni cytochrome b (cytb) gene. DNA was extracted from B. gibsoni isolated from Aomori Prefecture, Japan, and 1,288 basepairs of the cytb gene, including 1,071 basepairs of the open reading frame, were sequenced. The cytb gene of B. gibsoni obtained from three dogs that had been experimentally infected with B. gibsoni and treated with atovaquone was also sequenced. The B. gibsoni cytb gene obtained from all three atovaquone-treated dogs contained a single polymorphism resulting in an amino acid change in one of the putative ubiquinone-binding sites of Plasmodium falciparum. This polymorphism was homologous to mutations in other apicomplexan protozoa that exhibit resistance to atovaquone. Two other single polymorphisms were identified in parasites isolated from two of the dogs. These results indicate that single nucleotide polymorphisms in the sequence for mitochondrial cytb gene may be associated with decreased susceptibility of Babesia species to atovaquone.

Received June 27, 2005. Accepted for publication September 13, 2005.

Financial support: This study was supported by Grants-in-Aid for Scientific Research and Young Scientists from the Ministry of Education, Culture, Sports, Science and Technology of Japan, the Japan Society for the Promotion of Science, and the Kitasato University Research Grant for Young Researchers.

* Address correspondence to Aya Matsuu, Department of Small Animal Internal Medicine 1, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan. E-mail: matsuu{at}umas.kitasato-u.ac.jp

Authors’ addresses: Aya Matsuu, Kayoko Miyamoto, and Seiichi Higuchi, Department of Small Animal Internal Medicine 1, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan. Hiromi Ikadai, Department of Veterinary Parasitology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan. Shozo Okano, Department of Small Animal Surgery 3, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan. Telephone: 81-176-23-4371, E-mail: matsuu{at}umas.kitasato-u.ac.jp

Reprint requests: Aya Matsuu, Department of Small Animal Internal Medicine 1, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan, Telephone: 81-176-23-4371 ext 321, E-mail: matsuu{at}vmas.kitasato-u.ac.jp.






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Copyright © 2006 by the American Society of Tropical Medicine and Hygiene.