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Am. J. Trop. Med. Hyg., 74(3), 2006, pp. 348-352
Copyright © 2006 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


LIPOPOLYSACCHARIDE HETEROGENEITY AMONG BURKHOLDERIA PSEUDOMALLEI FROM DIFFERENT GEOGRAPHIC AND CLINICAL ORIGINS

NARISARA ANUNTAGOOL*, VANAPORN WUTHIEKANUN, NICHOLAS J. WHITE, BART J. CURRIE, RASANA W. SERMSWAN, SURASAKDI WONGRATANACHEEWIN, SUWIMOL TAWEECHAISUPAPONG, SANSANEE C. CHAIYAROJ, AND STITAYA SIRISINHA
Faculty of Tropical Medicine, and Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; University of Oxford, Oxford, United Kingdom; Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia; Northern Territory Clinical School, Flinders University, Darwin, Northern Territory, Australia; Melioidosis Research Center, Departments of Biochemistry and Microbiology, Faculty of Medicine, and Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen, Thailand

 

ABSTRACT

Heterogeneous patterns were obtained for lipopolysaccharide (LPS) from 1,327 Burkholderia pseudomallei isolates by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, silver staining, and immunoblot analysis. Two LPS serotypes (A and B) possessing different ladder profiles and a rough LPS without ladder appearances were identified. All three LPS types were antigenically distinct by immunoblotting. The predominant type A (97%) produced the lowest amount of biofilm. The two less common types (smooth type B and rough type) were found more in clinical than environmental isolates and more in Australian isolates than Thai isolates. These isolates were more often associated with relapse than with primary infection.

Received September 9, 2005. Accepted for publication October 19, 2005.

Acknowledgments: We thank Mark Mayo and Daniel Gal (Menzies School of Health Research, Darwin, Northern Territory, Australia) for assistance with isolate processing and some of the PFGE data mentioned. We also thank Prof. Nicholas PJ Day and Kasia Stepniewska (Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand) for their assistance in statistical analysis.

Financial support: This work was supported by grants RDG 4530209 and PDF/48/2544 from the Thailand Research Fund (Bangkok, Thailand) and the Wellcome Trust-Mahidol University-Oxford Tropical Medicine Research Programme funded by the Wellcome Trust of Great Britain, and by a project grant from the Australian National Health and Medical Research Council.

* Address correspondence to Narisara Anuntagool, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand. E-mail: narisara{at}tropmedres.ac

Authors’ addresses: Narisara Anuntagool, Vanaporn Wuthiekanun, and Nicholas J. White, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand, Telephone: 66-2-354-1389, Fax: 66-2-354-9169, E-mail: narisara{at}tropmedres.ac. Bart J. Currie, P.O. Box 41096 Casuarina, Northern Territory 0811, Australia, Telephone: 61-8-8922-8056, Fax: 61-8-8927-5287, E-mail: bart{at}menzies.edu.au. Rasana W. Sermswan, Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. Surasakdi Wongratanacheewin, Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand, Telephone: 66-4-336-3515, Fax: 66-4-334-8375, E-mail: sura_wng{at}kku.ac.th. Suwimol Taweechaisupapong, Deparment of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen 40002, Thailand. Sansanee C. Chaiyaroj and Stitaya Sirisinha, Deparment of Microbiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand, Telephone: 66-2-201-5675, Fax: 66-2-644-5411, E-mail: scssr{at}mahidol.ac.th.




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