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Am. J. Trop. Med. Hyg., 74(3), 2006, pp. 345-347
Copyright © 2006 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


PULSED-FIELD GEL ELECTROPHORESIS AS A DISCRIMINATORY TYPING TECHNIQUE FOR THE BIOTHREAT AGENT BURKHOLDERIA MALLEI

NARISARA CHANTRATITA, MONGKOL VESARATCHAVEST, VANAPORN WUTHIEKANUN, RACHANEEPORN TIYAWISUTSRI, TSEDEV ULZIITOGTOKH, ERHAN AKCAY, NICHOLAS P. J. DAY, AND SHARON J. PEACOCK*
Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Biocombinat, State-Owned Biological Enterprise, Ulaanbaatar, Mongolia; Tuberculosis Laboratory, Central Veterinary Control and Research Institute, Ankara, Turkey; Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical; Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom

 

ABSTRACT

Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) was used to type 21 laboratory strains of Burkholderia mallei. We demonstrated good resolution by PFGE together with clustering of some geographically related isolates, and confirmed previous observations that B. mallei is clonal as defined by MLST.

Received September 15, 2005. Accepted for publication October 19, 2005.

Acknowledgments: We thank the support of staff at the Wellcome Trust-Mahidol University-Oxford University Tropical Medicine Research Program for their assistance, and Professor Don Woods (University Calgary, Calgary, Alberta, Canada) for providing bacterial strains.

Financial support: This study was supported by the Wellcome Trust. Sharon J. Peacock is supported by a Wellcome Trust Career Development Award in Clinical Tropical Medicine.

* Address correspondence to Sharon J. Peacock, Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand. E-mail: sharon{at}tropmedres.ac

Authors’ addresses: Narisara Chantratita, Mongkol Vesaratchavest, Vanaporn Wuthiekanun, Rachaneeporn Tiyawisutsri, Nicholas P. J. Day, and Sharon J. Peacock, Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand, Telephone: 66-2-354-9172, Fax: 66-2-354-9169, E-mail: sharon{at}tropmedres.ac. Tsedev Ulziitogtokh, Biocombinat, State-Owned Biological Enterprise, Ulaanbaatar, Mongolia, E-mail: bio_info{at}mbox.mn. Erhan Akcay, Tuberculosis Laboratory, Central Veterinary Control and Research Institute, 06020 Etlik Ankara, Turkey, Telephone: 90-312-326-0090, Fax: 90-312-321-1755, E-mail: ehh.o{at}tr.net and etlik{at}vet.gov.tr.






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