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Am. J. Trop. Med. Hyg., 73(6), 2005, pp. 1162-1164
Copyright © 2005 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


APPLICATION OF A POLYMERASE CHAIN REACTION TO DETECT BURKHOLDERIA PSEUDOMALLEI IN CLINICAL SPECIMENS FROM PATIENTS WITH SUSPECTED MELIOIDOSIS

DANIEL GAL, MARK MAYO, EMMA SPENCER, ALLEN C. CHENG, AND BART J. CURRIE*
Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia; Infectious Diseases Unit, Northern Territory Clinical School, Flinders University, Royal Darwin Hospital, Darwin, Northern Territory, Australia

 

ABSTRACT

The diagnostic potential of a Burkholderia pseudomallei type three secretion system (TTS1) polymerase chain reaction (PCR) was examined on clinical specimens from 27 patients with sepsis in the Northern Territory of Australia, a region endemic for melioidosis. The TTS1 PCR was conducted on DNA extracted from a range of clinical specimens (blood, sputum, urine, joint, pericardial and pleural fluid, and swabs from skin lesions, throat, nose, and rectum). The PCR sensitivity in culture-positive clinical specimens from the nine confirmed patients with melioidosis was 65% and the specificity was 100%, with no PCR-positive results in specimens from 18 patients without melioidosis. The PCR based on the B. pseudomallei TTS1 has the potential to substantially improve the timeliness of diagnosis of melioidosis.

Received June 24, 2005. Accepted for publication August 10, 2005.

Acknowledgments: We are grateful to Dr. Gary Lum and the microbiology staff at Royal Darwin Hospital Pathology for culturing clinical specimens and identifying the bacteria. We also thank our clinical colleagues at Royal Darwin Hospital for their assistance in collecting patient specimens and Dr. Jay Gee (Meningitis and Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia) for providing the Qiagen kits.

Financial support: This study was supported by a Project Grant from the Australian National Health and Medical Research Council.

* Address correspondence to Bart J. Currie, Menzies School of Health Research, P.O. Box 41096, Casuarina, Northern Territory 0811 Australia. E-mail: bart{at}menzies.edu.au

Authors’ address: Daniel Gal, Mark Mayo, Emma Spencer, Allen C. Cheng, and Bart J. Currie, Menzies School of Health Research, P.O. Box 41096, Casuarina, Northern Territory 0811, Australia, Telephone: 61-8-8922-8196, Fax: 61-8-8927-5187, E-mail: bart{at}menzies.edu.au.

Reprint requests: Bart J. Currie, Menzies School of Health Research, P.O. Box 41096, Casuarina, Northern Territory 0811, Australia.




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