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Am. J. Trop. Med. Hyg., 73(5), 2005, pp. 962-963
Copyright © 2005 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


ENUMERATING LEPTOSPIRES USING THE COULTER COUNTER

CHRISTINA M. HUMBERD*, CLINTON K. MURRAY, SAUNDRA K. STUART, BARBARA A. REEB, AND DUANE R. HOSPENTHAL
Infectious Diseases Department, Wilford Hall USAF Medical Center, San Antonio, Texas; the Department of Medicine and Department of Clinical Investigation, Brooke Army Medical Center, Fort Sam Houston, Texas

 

ABSTRACT

The currently accepted gold standard for enumeration of leptospires is tabulation of organisms using a Petroff-Hausser counting chamber and dark-field microscopy, a technically demanding, time-consuming technique. Quantification of leptospires with a Coulter counter produced reliable and reproducible counts, comparable to the counting chamber. This experiment demonstrates that the faster, less technically demanding Coulter counter may be an alternative to determine numbers of leptospires.

Received April 26, 2005. Accepted for publication May 27, 2005.

Disclaimer: The views expressed herein are those of the authors and do not reflect the official policy or position of the Department of the Army, the Department of the Air Force, Department of Defense, or the U.S. Government. The authors are employees of the U.S. government. This work was prepared as part of their official duties and, as such, there is no copyright to be transferred.

* Address correspondence to Christina M. Humberd, USAF, MC Infectious Disease (759 MDOS/MMII), Wilford Hall Medical Center, 2200 Bergquist Drive, Suite 1, Lackland AFB, TX 78236-9908. E-mail: Christina.Humberd{at}lackland.af.mil

Authors’ addresses: MAJ Clinton K. Murray, MC, USA, Infectious Disease (MCHE-MDI), Brooke Army Medical Center, 3851 Roger Brooke Drive, Fort Sam Houston, TX 78234, Telephone: (210) 916-4355, Fax: (210) 916-0388, E-mail: Clinton.Murray{at}amedd.army.mil. Capt Christina M. Humberd, USAF, MC, Infectious Disease (759 MDOS/MMII), Wilford Hall Medical Center, 2200 Bergquist Drive, Suite 1, Lackland AFB, TX 78236-9908, Telephone: (210) 292-6726, Fax: (210) 292-3740, E-mail: Christina.Humberd{at}lackland.af.mil. Saundra K. Stuart and Barbara A. Reeb, Department of Clinical Investigation (MCHE-DCI), Brooke Army Medical Center, 3851 Roger Brooke Drive, Fort Sam Houston, TX 78234, Telephone: (210) 916-4355, Fax: (210) 916-0388. LTC Duane R. Hospenthal, MC, USA, Infectious Disease (MCHE-MDI), Brooke Army Medical Center, 3851 Roger Brooke Drive, Fort Sam Houston, TX 78234, Telephone: (210) 916-4355, Fax: (210) 916-0388.

Reprint requests: MAJ Clinton K. Murray, MC, USA, Infectious Disease (MCHE-MDI), Brooke Army Medical Center, 3851 Roger Brooke Drive, Fort Sam Houston, TX 78234, Telephone: (210) 916-4355, Fax: (210) 916-0388, E-mail: Clinton.Murray{at}amedd.army.mil.






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Copyright © 2005 by the American Society of Tropical Medicine and Hygiene.