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The assay of choice for serological detection of cysticercosis in humans and pigs is the enzyme-linked immunoelectrotransfer blot (EITB), a Western blot assay that relies on the use of seven lentil-lectin–purified glycoproteins (LLGPs) derived from Taenia solium metacestodes. The EITB is has a sensitivity of 98% and a specificity of 100% in detecting cysticercosis, yet scarcity of native source material and the labor-intensive process of metacestode purification hinder its practicality. These limitations have necessitated the reproduction of the EITB antigens in synthetic forms. Four chemically synthesized LLGP antigens, TS14, TS18var1, TSRS1, and TSRS2var1, were assayed individually by enzyme-linked immunosorbent assay (ELISA) and Western blot for immunoreactivity against a large cohort of sera from clinically defined neurocysticercosis patients. The sensitivity and specificity of all four of these antigens using the ELISA format were well below the standards set by the LLGP EITB, whereas results of the Western blot format closely mirrored those of the LLGP EITB.
Received February 3, 2005. Accepted for publication May 19, 2005.
Financial support: This research was supported by the International Collaborations in Infectious Disease Research–National Institutes of Health (NIH) grant U01 A135894, NIH National Institute of Allergy and Infectious Diseases Tropical Medicine Research Center grant 1 P01 AI51976-01, Wellcome grant 063109, and Bill and Melinda Gates Foundation grant 23981.
* Address correspondence to Christina M. Scheel, Division of Parasitic Diseases, Centers for Disease Control and Prevention, Building 23, Room 1003, Mailstop F-13, 4770 Buford Highway, Atlanta, GA 30341-3717. E-mail: cmscheel{at}aol.com
Other members of The Cysticercosis Working Group in Peru include H. Mayta (Universidad Peruana Cayetano Heredia, Lima, Peru), M. T. Lopez and M. Silva (Universidad Nacional Mayor de San Marcos, Lima, Peru), S. Rodriguez (Instituto Nacional de Ciencias Neurologicas, Lima, Peru), and J. Noh (Centers for Disease Control and Prevention, Atlanta, GA).
Authors’ addresses: Christina M. Scheel, Azra Khan, Kathy Hancock, and Victor C. W. Tsang, Division of Parasitic Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, Building 23, Room 1003, Mail Stop F-13, Atlanta, GA 30341-3717, Telephone: 770-488-4056, Fax: 770-488-4109, E-mails: cmscheel{at}aol.com, amk7{at}cdc.gov, kyh7{at}cdc.gov, and vct1{at}cdc.gov. Hector H. Garcia, Department of Microbiology, Universidad Peruana Cayetano Heredia, Avenida Honorio Delgado 430, San Martin de Porras, Lima, Peru, Telephone: 51-1-328-7360, Fax: 51-1-328-4038, E-mail: hgarcia{at}terra.com.pe. Armando E. Gonzalez, Department of Veterinary Public Health, School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Cuadra 29 Avenida Circunvalacion s/n, San Borja, Lima, Peru, Telephone: 51-1-436-8938, Fax: 51-1-448-8931, E-mail: emico{at}terra.com.pe. Robert H. Gilman, Department of International Health, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, Telephone: 410-614-3639, Fax: 410-614-6060, E-mail: rgilman{at}jhsph.edu.
Reprint requests: Victor C. W. Tsang, Centers for Disease Control, 4770 Buford Highway, Building 23, Room 1003, Mail Stop F-13, Atlanta, Georgia 30341-3717. Telephone: 770-488-4056. Fax: 770-488-4109.
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  I. Mamkin, N. Sood, and S. V. Ramanan Taenia solium Neurocysticercosis N. Engl. J. Med., October 18, 2007; 357(16): 1666 - 1667. [Full Text] [PDF]
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