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Am. J. Trop. Med. Hyg., 73(3), 2005, pp. 517-519
Copyright © 2005 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


TRYPANOSOMA CRUZI INFECTION IN WILD MAMMALS FROM A CHAGASIC AREA OF CHILE

MARLENE ROZAS, CAREZZA BOTTO-MAHAN*, XIMENA CORONADO, SYLVIA ORTIZ, PEDRO E. CATTAN, AND ALDO SOLARI
Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile; Department of Ecological Sciences, Faculty of Sciences, University of Chile, Santiago, Chile; Department of Biological Sciences, Faculty of Veterinary Sciences, University of Chile, Santiago, Chile

 

ABSTRACT

We report results of PCR-DNA based detection of Trypanosoma cruzi in wild mammal reservoirs from a chagasic area of Chile. We analyzed 157 blood samples from wild mammals including the marsupial Thylamis elegans and the rodents Octodon degus, Phyllotis darwini, and Abrothrix olivaceus. In addition, 42 blood samples from goats (i.e., a peridomestic mammal) were analyzed. Blood samples were used to extract DNA, and PCR was performed using the amplification of minicircle DNA sequences. Southern analysis was used to confirm diagnosis with a universal probe of P32-labeled kinetoplast DNA. Altogether, 51% of the wild and 36% of the peridomestic mammals were infected with T. cruzi. These findings suggest that the real T. cruzi infection levels in wild and peridomiciliary reservoirs are higher than those previously determined with serological and parasitological conventional methods. The relevance of our results is discussed in term of the risk factor of infection in human.

Received January 17, 2005. Accepted for publication April 6, 2005.

Financial support: This study was supported by grants FONDECYT 104-0762 and IAEA CHI-11762 to A.S. Additional support was obtained from the following grants: FONDECYT 2010022 and 3050033 (C.B.M.) and 1040711 (P.E.C.).

* Address correspondence to Carezza Botto-Mahan, Department of Ecological Sciences, Faculty of Sciences, University of Chile, Casilla 653, Santiago, Chile. E-mail: cbotto{at}uchile.cl

Authors’ addresses: Marlene Rozas, Ximena Coronado, Sylvia Ortiz, and Aldo Solari, Program of Cellular and Molecular Biology, Faculty of Medicine, University of Chile, Casilla 70086, Santiago 7, Chile, Telephone: 56-2-6786062, Fax: 56-2-735-5580, E-mail: asolari{at}machi.med.uchile.cl. Carezza Botto-Mahan, Department of Ecological Sciences, Faculty of Sciences, University of Chile, Casilla 653, Santiago, Chile, Telephone: 56-2-6787232, Fax: 56-2-272-7363, E-mail: cbotto{at}uchile.cl. Pedro E. Cattan, Department of Biological Sciences, Faculty of Veterinary Sciences, University of Chile, Casilla 2 correo 15, Santiago, Chile, Telephone: 56-2-6785629, Fax: 56-2-6785526, E-mail: pcattan{at}uchile.cl.




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Copyright © 2005 by the American Society of Tropical Medicine and Hygiene.