AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
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Am. J. Trop. Med. Hyg., 72(6), 2005, pp. 714-718
Copyright © 2005 by The American Society of Tropical Medicine and Hygiene

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CHARACTERIZATION OF A HUMAN REFERENCE STANDARD FOR ANTIBODY TO PLASMODIUM FALCIPARUM MEROZOITE SURFACE PROTEIN 142

IN-KYU YOON, EVELINA ANGOV, DAVID LARSON, D. GRAY HEPPNER, JAMES F. CUMMINGS, AND V. ANN STEWART
Division of Communicable Diseases and Immunology, Department of Immunology, Walter Reed Army Institute of Research, Silver Spring, Maryland

Volunteers vaccinated with a candidate malaria vaccine containing merozoite surface protein 142 (MSP-142) exhibit antibodies to MSP-142 that are measured by enzyme-linked immunosorbent assay (ELISA). The purpose of this study was to make a human reference standard for MSP-142 antibody measured in absolute quantity units using pooled plasma samples known to contain high titers of MSP-142 antibody based on previous ELISA results. Immobilized metal affinity chromatography was used to determine the amount of MSP-142 antibody in this plasma pool. Hexahistidine-tagged MSP-142 antigen adsorbed to nickel-chelating resin was used to capture MSP-142 antibody from the plasma pool. The intact MSP-142 antibody-antigen complexes were eluted and total IgG was measured by an ELISA standardized against purified human IgG. In this way, the human reference standard was determined to contain 48.3 µg/mL of MSP-142 antibody. This reference standard may be useful as a quantitative working standard for measuring MSP-142 antibody response in future vaccine clinical trials involving MSP-1.

Received August 11, 2004. Accepted for publication October 7, 2004.

Disclaimer: The views of the authors are private and are not to be construed as official policy of the Department of Defense or the U.S. Army.

Authors’ addresses: In-Kyu Yoon, Department of Allergy-Immunology, Walter Reed Army Medical Center, 6900 Georgia Avenue, Washington, DC 20307, Telephone: 202-782-8074, Fax: 202-782-7093, E-mail: in-kyu.yoon{at}na.amedd.army.mil. Evelina Angov, David Larson, D. Gray Heppner, and V. Ann Stewart, Division of Communicable Diseases and Immunology, Department of Immunology, Walter Reed Army Medical Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, E-mails: evelina.angov{at}na.amedd.army.mil, david.larson{at}na.amedd.army.mil, donald.heppner{at}na.amedd.army.mil, and ann.stewart{at}na.amedd.army.mil. James F. Cummings, Infectious Disease Service, Walter Reed Army Medical Center, 6900 Georgia Avenue, Washington, DC 20307, E-mail: james.cummings{at}na.amedd.army.mil.






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Copyright © 2005 by the American Society of Tropical Medicine and Hygiene.