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STRONGYLOIDES STERCORALIS RECOMBINANT NIE ANTIGEN SHARES EPITOPE WITH RECOMBINANT Ves v 5 AND Pol a 5 ALLERGENS OF INSECTS

A new recombinant protein (NIE) for immunodiagnosis of human Strongyloides infection has 13% to 18% amino acid identity with antigen 5 insect venom allergen, but the C-terminal segment of NIE showed highest identity with Ves v 5 (yellow jacket) and Pol a 5 (paper wasp). A rabbit polyclonal anti-NIE antibody identified a single band of NIE antigen as well as bands of Pol a 5 and Ves v 5 antigens, and mouse anti-Pol a 5 and anti-Ves v 5 sera reacted with recombinant NIE antigen by Western blot. A cyanogen bromide-digested C-terminal fragment of NIE was reactive with mouse anti-Ves v 5 and Pol a 5 antibodies as well as with rabbit anti-NIE serum. Although IgE and IgG antibodies from pooled sera from Strongyloides-infected patients reacted with Pol a 5 and Ves v 5 recombinant antigens on immunoblots, neither antigen inhibited human IgG reaction with NIE antigen in a competitive enzyme-linked immunosorbent assay.

Received July 8, 2004. Accepted for publication October 22, 2004.

Acknowledgments: The authors thank Mark Garfield for help with N-terminal amino acid sequencing of NIE antigen by Edman degradation and Brenda Rae Marshall for help preparing this manuscript.

Authors’ addresses: Varatharajalu Ravi, Laboratory of Parasitic Diseases (LPD), National Institute of Allergy and Infectious Diseases (NIAID), 4 Center Drive, Room 4/126, National Institutes of Health (NIH), Bethesda, MD 20892, Telephone: 301-496-9892, Fax: 301-402-0079, E-mail: rvaratharajalu{at}niaid.nih.gov. Te Piao King, Rockefeller University, New York, 1230 York Avenue, New York, NY 10021, Telephone: 212-327-8212, Fax: 212-327-8878, E-mail: kingtp{at}mail.rockefeller.edu. John F. Andersen, LPD, NIAID, 4 Center Drive, Room 4/126, NIH, Bethesda, MD 20892, Telephone: 301-435-2967, Fax: 301-402-2201, E-mail: jandersen{at}niaid.nih.gov. Thomas B. Nutman, LPD, NIAID, 4 Center Drive, Room 4/126, NIH, Bethesda, MD 208923, Telephone: 301-496-5398, Fax: 301-480-3757, E-mail: tnutman{at}niaid.nih.gov. Franklin A. Neva, LPD, NIAID, 4 Center Drive, Room 4/126, NIH, Bethesda, MD 20892, Telephone: 301-496-2486, Fax: 301-402-0079, E-mail: fneva{at}niaid.nih.gov.

Reprint requests: Franklin A. Neva, LPD, NIAID, 4 Center Drive, Room 4/126, NIH, Bethesda, MD 20892, Telephone: 301-496-2486, Fax: 301-402-0079, E-mail: fneva{at}niaid.nih.gov.