| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
A hierarchical population genetic study was conducted among 19 Aedes aegypti populations in Thailand from Chiang Mai in the north to Songkhla province in the south. Single-strand conformation polymorphism analysis was used to examine variation in a 359-basepair region of the NADH dehydrogenase subunit 4 mitochondrial DNA gene (ND4). Seven haplotypes were detected in two lineages previously identified in ND4 haplotypes from North America. Gene flow estimates and highly significant variation among populations within 25 kilometers implicated genetic drift and vector control efforts as major factors in genetic structure. Mantel regression analysis demonstrated no isolation by distance. Urban areas were relatively panmictic, while suburban/rural sites exhibited more restricted gene flow. Significant genetic structure among groups of collections > 100 kilometers apart is consistent with recent (~50 year) expansion of Ae. aegypti from highly populated areas accompanied by founder effects, but could also reflect the overall low genetic diversity in ND4 in Thailand.
Received February 3, 2004. Accepted for publication August 5, 2004.
Acknowledgements: We greatly appreciate the cooperation of many citizens of Thailand for allowing us to collect mosquitoes from their homes. We thank Kuhn Samporn Chanaimongkol and Kuhn Pradith for assistance in making mosquito collections and William C. Black IV for advice on statistical analyses and critical reading of an earlier version of this manuscript.
Financial support: This research was supported by grant AI-22119 from the National Institutes of Health to Thomas W. Scott.
Authors’ addresses: Christopher F. Bosio, Department of Microbiology, Immunology and Pathology, 1682 Campus Delivery, Colorado State University, Fort Collins, CO 80523, Telephone: 970-491-4879, Fax: 970-491-1815, E-mail: Christopher.Bosio{at}colostate.edu. Laura C. Harrington, Department of Entomology, Cornell University, Ithaca, NY 14853, Telephone: 607-255-4475 Fax: 607-255-0939 E-mail: lch27{at}cornell.edu. James W. Jones, Department of Entomology, Armed Forces Research Institute for Medical Sciences, Bangkok, Thailand, Telephone: 66-2-644-6897, Fax: 66-2-246-8832, E-mail: jonesjw2{at}afrims.org. Ratana Sithiprasasna, Department of Entomology, Armed Forces Research Institute for Medical Sciences, Bangkok 10400, Thailand, Telephone: 66-2-644-5777, Fax: 66-2-246-8832, E-mail: ratanas{at}afrims.org. Douglas E. Norris, Department of Medical Microbiology and Immunology, Johns Hopkins University, Baltimore, MD 21205, Telephone: 410-614-2710, Fax: 410-955-0105, E-mail: dnorris{at}jhsph.edu. Thomas W. Scott, Department of Entomology, University of California, Davis CA 95616, Telephone: 530-754-4196, Fax: 530-752-1537, E-mail: twscott{at}ucdavis.edu.
This article has been cited by other articles:
|
|
 |
|
  V. M. Scarpassa, T. B. Cardoza, and R. P. Cardoso Junior Population Genetics and Phylogeography of Aedes aegypti (Diptera: Culicidae) from Brazil Am J Trop Med Hyg, June 1, 2008; 78(6): 895 - 903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Urdaneta-Marquez, C. Bosio, F. Herrera, Y. Rubio-Palis, M. Salasek, and W. C. Black IV Genetic Relationships among Aedes aegypti Collections in Venezuela as Determined by Mitochondrial DNA Variation and Nuclear Single Nucleotide Polymorphisms Am J Trop Med Hyg, March 1, 2008; 78(3): 479 - 491. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
