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Am. J. Trop. Med. Hyg., 72(4), 2005, pp. 415-420
Copyright © 2005 by The American Society of Tropical Medicine and Hygiene

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EVALUATION OF 7SL RNA GENE SEQUENCES FOR THE IDENTIFICATION OF LEISHMANIA SPP.

ADRIAN M. ZELAZNY, DANIEL P. FEDORKO, LI LI, FRANKLIN A. NEVA, AND STEVEN H. FISCHER
Microbiology Service, Department of Laboratory Medicine, Clinical Center, and Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland

We evaluated the use of 7SL RNA gene sequences for the identification of Leishmania spp. A fragment (~137 basepairs) of the 7SL RNA gene from 13 reference strains and 18 clinical isolates of 11 different Leishmania species was amplified and sequenced using conserved primers. Reference strains from each Leishmania spp. complex showed unique sequences. The nucleotide sequences were compared pairwise and a range of 81.0–99.3% intercomplex similarity was observed. Clinical isolates of the same species had sequences identical to the corresponding reference strains; thus, the intraspecies similarity was 100%. A phylogenetic tree derived from the 7SL RNA gene partial sequences was constructed and is in agreement with accepted phylogenetic schemes.

Received August 26, 2004. Accepted for publication November 24, 2004.

Acknowledgments: We thank Dennis M. Dwyer (Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health) for providing reference strains of L. tarentolae, C. fasciculata, and C. luciliae. We also thank Patrick R. Murray (Department of laboratory Medicine, Microbiology Service, Clinical Center, National Institutes of Health) for critically reviewing the manuscript.

Authors’ addresses: Adrian M. Zelazny, Daniel P. Fedorko, Li Li, and Steven H. Fischer, Microbiology Service, Department of Laboratory Medicine, Clinical Center, Building 10, Second Floor, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, Telephone: 301-496-4433, Fax: 301-402-1886, E-mails: azelazny{at}cc.nih.gov, dfedorko{at}cc.nih.gov, lli{at}cc.nih.gov and sfischer{at}cc.nih.gov. Franklin A. Neva, Opportunistic Diseases Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Building 4, Room B1-27, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, Telephone: 301-496-2486, Fax: 301-402-0079, E-mail: fneva{at}niaid.nih.gov.

Reprint requests: Steven H. Fischer, Microbiology Service, Department of Laboratory Medicine, Building 10, Room 2C-381B, National Institutes of Health 10 Center Drive, Bethesda, MD 20892.






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